3. Identify one advantage an organism might gain from expressing endogenous endonucleases. 4. You were the first person to discover a restriction enzyme in Xanthamonas badrii. Using the tradition schematie, what would you name this enzyme? 5. In Southern blotting, the "probe" is which type of molecule: DNA, RNA, or Protein? 6. Identify the following terms, as they relate to DNA cloning: A. Vector B. Competent C. Transformation D. Clone 7. Deseribe the function of restriction enzymes during cell-based DNA cloning. e Darad on the information

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Please help me with questions 3,5,7,8,9,10

1. Identify the following terms, as they relate to transcription:
A. Coding/Sense Strand
B. Non-Coding/Anti-Sense Strand
C. Functional directionality of RNA Polymerase (reading vs. building)
D. Splicing
2. Describe the differences in location and function between the codon and anticodon.
3. Identify one advantage an organism might gain from expressing endogenous
endonucleases.
4. You were the first person to discover a restriction enzyme in Xanthamonas badrii.
Using the tradition schematic, what would you name this enzyme?
5. In Southern blotting, the "probe" is which type of molecule: DNA, RNA, or Protein?
6. Identify the following terms, as they relate to DNA cloning:
A. Vector
B. Competent
C. Transformation
D. Clone
7 Describe the function of restriction enzymes during cell-based DNA cloning.
8. Based on the information given, design a 6-nucleotide-long primer set to amplify the indicated
portion of DNA:
5' GGTAGC GTGACAATGTGCGATGAGACGTAG 3
3'CCATCGCACTGTTACACGCTACTCTGCATC5'
9. Why would a researcher choose to use RT-PCR instead of regular PCR? What information
could he/she receive through this technique?
here to
10. Identify the structural difference between dNTPs and ddNTPs and the role this plays in DNA
sequencing
Transcribed Image Text:1. Identify the following terms, as they relate to transcription: A. Coding/Sense Strand B. Non-Coding/Anti-Sense Strand C. Functional directionality of RNA Polymerase (reading vs. building) D. Splicing 2. Describe the differences in location and function between the codon and anticodon. 3. Identify one advantage an organism might gain from expressing endogenous endonucleases. 4. You were the first person to discover a restriction enzyme in Xanthamonas badrii. Using the tradition schematic, what would you name this enzyme? 5. In Southern blotting, the "probe" is which type of molecule: DNA, RNA, or Protein? 6. Identify the following terms, as they relate to DNA cloning: A. Vector B. Competent C. Transformation D. Clone 7 Describe the function of restriction enzymes during cell-based DNA cloning. 8. Based on the information given, design a 6-nucleotide-long primer set to amplify the indicated portion of DNA: 5' GGTAGC GTGACAATGTGCGATGAGACGTAG 3 3'CCATCGCACTGTTACACGCTACTCTGCATC5' 9. Why would a researcher choose to use RT-PCR instead of regular PCR? What information could he/she receive through this technique? here to 10. Identify the structural difference between dNTPs and ddNTPs and the role this plays in DNA sequencing
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