1. Restriction enzymes are extensively used in molecular biology. Below are the recognition sites of two of these enzymes, BamHI and Bcll. a) Shown below is a BamHl recognition site, which cleaves after the first G. Does cleavage by BamHI result in a 5' or 3' overhang? 5... GGATCC...3 3...CCTAGG...5 b) Shown below is a Bcll recognition site, which cleaves cleaves after the first T. Does cleavage by Bcll result in a 5' or 3' overhang? 5... TGATCA...3" 3... ACTAGT...5 You are given the DNA shown below. 5' ATTGAGGATCCGTAATGTGTCCTGATCACGCTCCACG 3' 3' TAACTCCTAGGCATTACACAGGACTAGTGCGAGGTGC 5' c) If the above DNA was cut with BamHI, how many DNA fragments would you expect? Write out the sequence of these double-stranded fragments.

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1. Restriction enzymes are extensively used in molecular biology. Below are the recognition sites of
two of these enzymes, BamHl and Bcll.
a) Shown below is a BamHI recognition site, which cleaves after the first G. Does cleavage by
BamHl result in a 5' or 3' overhang?
5... G'GATCC...3
3...CCTAGG...5
b) Shown below is a Bcll recognition site, which cleaves cleaves after the first T. Does cleavage by
Bcll result in a 5' or 3' overhang?
5... TGATCA...3
3... ACTAGT...5
You are given the DNA shown below.
5' ATTGAGGATCCGTAATGTGTCCTGATCACGCTCCACG 3'
3' TAACTCCTAGGCATTACACAGGACTAGTGCGAGGTGC 5'
c) If the above DNA was cut with BamHI, how many DNA fragments would you expect? Write out
the sequence of these double-stranded fragments.
d) If the above DNA was cut with Bcll, how many DNA fragments would you expect? Write out the
sequence of these double-stranded fragments.
e) If you ligate or join the smaller restriction fragment in step c with the smaller restriction
fragment in step d, what would be the sequence of the resulting recombinant DNA fragment?
Could you cut this recombinant DNA fragment with either BamH1 or Bcll? Yes or No, explain
f)
briefly.
Transcribed Image Text:1. Restriction enzymes are extensively used in molecular biology. Below are the recognition sites of two of these enzymes, BamHl and Bcll. a) Shown below is a BamHI recognition site, which cleaves after the first G. Does cleavage by BamHl result in a 5' or 3' overhang? 5... G'GATCC...3 3...CCTAGG...5 b) Shown below is a Bcll recognition site, which cleaves cleaves after the first T. Does cleavage by Bcll result in a 5' or 3' overhang? 5... TGATCA...3 3... ACTAGT...5 You are given the DNA shown below. 5' ATTGAGGATCCGTAATGTGTCCTGATCACGCTCCACG 3' 3' TAACTCCTAGGCATTACACAGGACTAGTGCGAGGTGC 5' c) If the above DNA was cut with BamHI, how many DNA fragments would you expect? Write out the sequence of these double-stranded fragments. d) If the above DNA was cut with Bcll, how many DNA fragments would you expect? Write out the sequence of these double-stranded fragments. e) If you ligate or join the smaller restriction fragment in step c with the smaller restriction fragment in step d, what would be the sequence of the resulting recombinant DNA fragment? Could you cut this recombinant DNA fragment with either BamH1 or Bcll? Yes or No, explain f) briefly.
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