1. You are having problems getting good transfection efficiency when you put your plasmid in e. coli. You want to freeze down a stock of e. coli that is more than 99% transfected. What modifications can you make to your plasmid and what steps can you take to ensure that 99% of your e. coli are transfected?

1. You are having problems getting good transfection efficiency when you put your plasmid in e. coli. You want to freeze down a stock of e. coli that is more than 99% transfected. What modifications can you make to your plasmid and what steps can you take to ensure that 99% of your e. coli are transfected?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...

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You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid is present in 100 copies per E. coli cell. You aim to have a final plasmid concentration of 100 ng/µl in a total volume of 50 µl. Assuming the yield is 100 %, how many E. coli cells should the culture from which the plasmid is to be isolated at least contain
4 I.I 5 | I6 I Use the following information to answer questions 14-15. A student has forgotten which antibiotic plasmid she used in her E. coli transformation. It could have been kanamycin, ampicillin, or tetracycline. She decides to make up a special set of plates to determine the type of antibiotic used. The plates below show the results of the test. Plate I. LB agar with kanamycin Plate II. LB agar with ampicillin Plate III. LB agar with tetracycline Plate IV. LB agar 14) Which antibiotic plasmid has been used? (kanamycin, ampicillin or tetracycline?) (a, b, c or d?) 15) Which of the following choices is best explanation for these results?
What do you mean by vector plasmid?
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
1 2. 3. Two different cultures of Escherichia coli bacteria were inoculated onto nutrient agar plates. One culture was transformed with pGLO plasmid and the other culture was not. All of the plates shown above are illuminated with ultraviolet light. • Which plate was inoculated with E.coli+PGLO bacteria and included ampicillin antibiotic but did not include arabinose? • Which plate was inoculated with E.coli+pGLO bacteria and included both ampicillin antibiotic and arabinose. • What would you expect to observe on a plate that included ampicillin and was inoculated with E. coli minus (without) PGLO plasmid?
what are the plasmid status of bacterial cells resulting from conjugation between a f+ and a f- bacterium ? * a-Two F+ bacteria b-Two F- bacteria c-The F+ bacterium become F- and the F- bacterium become F+ d- The F+ bacterium remain as F+ , and the F- bacterium remain as F-
The presence of colonies on the bacterial plate post transformation suggests which of the following? Group of answer choices The colonies contain the transformed plasmid and are not resistant to the antibiotic The colonies contain the transformed plasmid and are resistant to the antibiotic The colonies do not contain the transformed plasmid and are not resistant to the antibiotic The colonies do not contain the transformed plasmid and are resistant to the antibiotic
Which of the following would be a good application of plasmid transformation? 1) to make copies of DNA 2) to isolate a change in a single nucleotide 3) to separate DNA fragments 4) to sequence DNA
EDTA weakens the cell wall by removing ions that help hold it together while glucose prevents premature cell lysis due to osmosis in cells with weakened cell walls. You have made a batch of resuspension solution to isolate plasmid DNA, but you forgot to add EDTA. What do you think will happen when you try to perform a plasmid isolation procedure with this reagent? Why?
Describe the 3 ways in which bacteria can transfer genetic material to each other via horizontal gene transfer. Be sure to explain the following: How is DNA transferred between bacteria, is there direct contact between bacteria and whether a plasmid is involved or not.
How can i explain my answers in depth in microbology for example this question: Assume that there are horizontal gene transfers between two completely different bacterial species. In one case it is a plasmid that is transferred via conjugation, in the other case it is a part of the bacterial chromosome that is transferred via transformation. In which of the two cases is it most likely that the transferred DNA will remain and be able to function in the recipient cells? Explain the biological background to your answer . How do I break down the question so I answer it fully
1)What is plasmid? 2) How do you explain the selection of plasmid for carrying the desired gene? 3) Follow the steps of human insulin production 4) Make a diagram for the production of growth hormone