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- 1. List as many as possible blood bank serologic tests that are affected by hemolyzed samples (list as many as possible and how) 2. Enumerate and describe the sources of antibodies utilized in the determination of blood group soluble substances.1. What is an antiserum? 2. What are the potency requirements in an antiserum? 3. What kind of antigen will anti-A detect? Anti-B? 4. Enumerate the common causes of false positive and false negative result in ABO forward grouping? 5. Give the purpose of Blood typing. 6. Cite the biochemical components of the ABO blood group accordingly. 7. Complete the table below Blood type A B 0 AB (positive/pos) for agglutination (negative/neg) for no agglutination Anti-A Anti-B1. Briefly describe the principle of erythrocyte rate (ESR) test. 2. Discuss five factors that influence the rate of red blood cell sedimentation 3. Indicate whether the ESR is increased or decreased in each of the following conditions Bellow;i. when sickle cells are presentii. increased environmental temperatureiii. unlevelled ESR rackiv. Vibrationv. Elevated plasma haemoglobinvi. leukocytosis
- 1. What are the indicators of remission in systemic lupus erythematosus (SLE)? Is it the normalization of erythrocyte sedimentation rate (ESR) and the disappearance of antinuclear antibodies (ANA) and other antibodies, or is it clinical improvement? 2. During remission of SLE, do ANA and other antibodies disappear?3. Please complete the table below by providing what is being asked. Supply the missing information needed. Blood Cell Image Physiological Role • (Insert role/function #1) (Insert role/function #2) • (Normal human RBC range million cells/uL) Erythrocyte [insert photo] (Insert role/function #1) • (Insert role/function #2) • (Normal human RBC range million cells/uL) Neutrophil [insert photo] Eosinophil [insert photo] • (Insert role/function #1) (Insert role/function #2) • (Normal human RBC range million cells/uL)2. Explain how endoreduplication/ endomitosis take place. 3. Identify the peculiarity in the maturation and development of megakaryocytic cells from the other cell series (erythrocytic, granulocytic, lymphocytic). 4. Describe the morphology of a mature platelet, life span, kinetics, normal/reference values. 5. Differentiate the 4 zones in a mature platelet by explaining the function of each.
- 3. At what age or time in life does an individual acquire the antibodies against ABO antigensother than their own? 4. At what age or time in life does an individual acquire antibodies against Rh positive blood if their own blood type is Rh negative? How do you explain this? 5. Does the vial labeled anti-A antiserum contain whole blood? Explain. What about the vials labeled anti-B and anti-Rh antiserum? 6. What is erythroblastosis fetalis and what is the cause? What blood type must the mother, father and child be in order for this to occur?1. Describe the three phases of the normal blood-clotting process. 2. Describe the antigens associated with the blood types of the ABO blood group.2. Cite at least three disorders associated with: a. Intrinsic Pathway b. Extrinsic Pathway 3. Illustrate and label the different steps of Partial Thromboplastin Time
- 1. Give the advantages of using tube method over the slide method. Why do you have to incubate the tube method in Rh typing? 2. Give the clinical importance of determining the Rh status of a patient? 3. Define HDN and explain the effect of Rh(D) antigen. What is the preventive measure of preventing D-negative mothers from forming anti-D? 4. What are the symptoms in Rh-mediated hemolytic transfusion reactions?8. Using ETS, coagulation tubes should be taken before plastic serum tubes as the clot activator in plastic serum tubes may interfere. Which of the following test would be most directly be affected? O A. Erythrocyte sedimentation rate O B. Glycosylated hemoglobin O C. Activated partial thromboplastin time O D. Serological assays1. If the MCHC value is decreased, what term is used to describe the peripheral blood smear finding? 2. Aside from automated and microhematocrit determinations, what red blood cell index can be used for indirect measurement of hematocrit? 3. Automated and microhematocrit determinations present discrepancy in the results due to what factor? 4. What is the wavelength used in the hemiglobincyanide method of hemoglobin determination? (Answer in this format: numerical value space abbreviated unit)