True or False Immobilization improves the stability of the enzyme. EnaLne, has a half-life of 10 days in free solution, but under identical conditions of temperature, pH, and medium composition, the measured half-life of a packed column is 30 days. The enzyme is immobilized in a porous sphere 5 mm in diameter.

Q: How does the optimum pH and optimum temperature can affect the enzyme function? Explain shortly with…

A: Temperature: Raising temperature generally speeds up a reaction, and lowering temperature slows down…

Q: Competitive and non-competitive inhibitions

A: Enzyme inhibition takes place when an inhibitor binds to the substrate and inhibit the activity of…

Q: True/False Question: Enzymes perform catalysis by selectively increasing the rates of the forward…

A: Enzymes are proteins that act as biological catalysts, that increase the rate of a chemical reaction…

Q: In First order, the rate of reaction would be . ., if the concertation of an enzyme is increased by…

A: The reaction may be classified according to the order of reaction, which is the number of reacting…

Q: the enzyme and substrate should be mixed only when the reaction is initiated (when the absorbance…

A: Kinetics is the branch which deals with rate of changes. From above all given situations in the…

Q: In covalent catalysis, either nucleophilic catalysis or electrophilic catalysis occurs. Do you agree…

A: Enzymes are known as biological catalysts, which perform an essential role in various biochemical…

Q: How many molecules of urea can one molecule of urease act on in 12.0 min ?

A: Number of molecules of urea in one molecule or urease act on in 12.0 min is given below in the 2nd…

Q: You have performed protein purification on your new favorite enzyme using a protocol which involves…

A: Proteins are formed of amino acid chains. They differ in molecular size, electric charge,…

Q: Based on your knowledge of the characteristics of enzymes, explain the practical and effective ways…

A: Biological washing powders are used to break the fats,grease,oil, blood stains,egg stains or any…

Q: The role of an enzyme in an enzyme-catalyzed reaction is to make the reaction more thermodynamically…

A: The enzyme-catalyzed reaction involves the enzyme, which helps in the conversion of substrate into…

Q: isoenzyme, specific enzyme activity, what is it? Briefly explain.

A: Enzymes are proteins with a specific globular shape. Enzymes contain an active site to which a…

Q: True or False. If drug A produces a larger zone of inhibition than drug B on the Kirby-Bauer disk…

A: Introduction If drug A does produce a larger zone of inhibition, that is one positive attribute of…

Q: Knowing that there is usually an optimum pH for enzyme activity, what do you think would happen if…

A: The pH scale is used to measure the acidity or alkalinity of a molecule and change in pH of a…

Q: Chain Shortening of monosaccharides can be done in the laboratory thru Kiliani-Fischer reaction.…

A: Carbohydrate chain shorting and lengthening is the process of increasing or decreasing the length of…

Q: Two different preparations of the same enzyme are characterized below. Step Volume Total protein…

A: The specific activity of an enzyme is usually calculated as activity divided by the weight of the…

Q: How does the optimum pH and optimum temperature can affect the enzyme function? Explain with…

A: Enzymes are biocatalysts that can increase the rate of biochemical reaction without themselves being…

Q: + H2O NH + HCO,- NH, Urea a. =NH HN =NH HN H2O + HCO,- H2N `CO, H,N b. L-Histidine L-Histamine c.…

A: Hi. Thank you for the question, As per the honor code, we are allowed to answer three sub-parts at a…

Q: Give a complete and well descriptive definition of the following: 1.1 Enzyme catalysis 1.2…

A: Enzymes are biocatalysts that are known to catalyze more than 5000 types of biochemical reactions.…

Q: Enzyme cofactors are usually inorganic ions that enhanceenzymatic activity by improving the “fit”…

A: True.

Q: List three effects of macromolecular crowding on the properties of enzymes and the reactions they…

A: Introduction: The organic molecules that are necessary for the growth and development of the body…

Q: A variety of factors influence enzyme activity. Substances that bind to the enzyme and interfere…

A: Enzymes are biocatalyst that increases the rate of reaction. Enzyme inhibition can be of 5 types :-…

Q: A plot of 1/Vo versus 1/[S], called Lineweaver-Burk or double-reciprocal plot, is a useful tool for…

A: Lineweaver-Burk plot is also known as the double reciprocal plot. Plot between the reciprocal of…

Q: Which of the following statements about the dUTP substrate for a dUTPase is true?

A: Option 2 is correct. The dUMP product is more easily formed when catalyzed by the dUTPase because…

Q: In the time course experiment, you will perform the incubations of 5 mL of the reaction mixtures of…

A: Add the 5ml of reaction mixture of 3 different enzymes in tripilicate in 10 ml test tubes. Add 5 ml…

Q: What effect does the optimum pH and temperature have on enzyme function? Briefly explain with an…

A: An enzyme is usually a proteinaceous substance that catalyzes a biological reaction. It is hence…

Q: An enzyme will work best in its optimum ( most favorable) pH, which is different for every enzyme.…

A: Changes in pH have an impact on enzymes. The optimal pH is the most beneficial pH value - the point…

Q: 8. Optimum (BEST) temperature for the action of this enzyme (graph below) is approximately 1. 50 1.…

A: Enzymes are very specific for their substrates and reaction conditions. These function at a specific…

Q: Since KM is an intrinsic property of an enzyme, its value does not depend on the enzyme…

A: Km is the Michaelis constant. It is defined as the substrate concentration at which half of the Vmax…

Q: Size Size Crude Anion Cation 2) Exclusion Exclusion Lysate Exchange Exchange 1 2 Total Protein…

A: Enzymes are vital to life and constitute one of the human body's most significant protein groups. A…

Q: If you multiplied enzyme concentration by kcat, think about what you would get. Which one of the…

A: The study of the in-vitro and in-vivo chemical reactions which occur in the biological system and…

Q: In the induced-fit model of enzyme action, the active site on the enzyme provides an exact fit for…

A: Enzyme are substances that catalyze chemical reactions. They bind to reactant molecules, called…

Q: Calculate your dilution strategyto make a 625 ng/mLHRP solution from a 12.5 μg/mL stock given the…

A: Final solution that has to be prepared: 625 ng/mL of HRP solution. Stock solution given: 12.5 μg/mL…

Q: Several factors contribute to enzyme catalysis. What arethey? Briefly explain the effect of each.

A: Enzymes are proteins that act as biocatalysts in speeding up biochemical reactions without actually…

Q: In concerted model, all subunits in the enzyme are either in the low affinity or high affinity…

A: There are two models of allosteric regulation of enzymes:-  - concerted model -sequential model

Q: Four important properties of enzymes are high catalytic rate,high degree of substrate specificity,…

A: Enzymes are known as biological catalysts, which perform an essential role in various biochemical…

Q: If an enzyme has a maximal activity of pH 6.8 and your reaction buffer is a pH 5.8, what do you need…

A: Every Enzyme has an optimum pH range for its maximuim acitivity. change in the pH will affect the…

Q: Which of the following amino acid residues cannot play a role in acid-base catalysis? a. Aspartate…

A: Acid-Base Catalysis is reaction process involving the movement of a proton from one molecule to…

Q: Which of the following statements regarding enzyme catalysis is false? All options are false. Once…

A: Normally an energy barrier exists between substrates (S) and products (P) i.e., S -> P…

Q: In a biochemical reaction that involves the substrate urea and the liver enzyme urease, decreasing…

A:

Q: Describe how you would make the following solution containing two enzymes.             1 mL total…

A: Molarity is the way of expressing the concentration of solute in the solution. It is given by the…

Q: Km value of an enzyme

A: The study of the rates of enzyme-catalyzed chemical reactions is known as enzyme kinetics. here they…

Q: "Enzyme immobilization confers greater stability to the enzyme"-Do you agree or disagree with the…

A: Introduction: Enzymes are biological catalyst that fastens the rate of chemical reactions. It is…

Q: Describe the reactants and products of the following enzyme catalyzed reaction. Names of molecules…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: How many moles of active sites are there in 1 mg of enzyme? Assume that each subunit has one active…

A: Enzyme : It are proteins that act as biological catalysts. Catalysts accelerate chemical reactions.…

Q: The membrane proteins are mostly glycoproteins in association with enzymes to hasten the biochemical…

A: Introduction: The biological membranes that separate the cell inside from the outside environment…

Q: Based on the relative increase in purity, place the purification procedures used for this enzyme in…

A: Hi! Thank you for the question. We are authorized to answer one question at a time, since you have…

Q: List down the properties of an ideal carrier matrix for enzyme immobilization.

A: Enzymes can be used as catalytic agents in medical and industrial applications. Such enzymes are…

Q: The factors that denature enzyme activity are temperature, pH, salinity, alcohol and surface area.

A: Enzyme are biocatalyst and protenious in nature. Enzyme act on substrate molecules and convert to…

Q: EFFECT OF pH ON ENZYME ACTIVITY Results: Tube 1 Tube 2 Tube 3 Time in Minutes Iodine Benedict's…

A: Iodine test determines the presence of starch in the sample by turning into blue black colour.…