the enzyme and substrate should be mixed only when the reaction is initiated (when the absorbance readings are taken) the michaelis constant must be known
Q: Given Tagatose, Briefly explain its expected reaction (based on their structural formula) to the…
A: The Molisch's test, Fehling's test, and Bial's test are the qualitative tests for carbohydrates. In…
Q: Enzymes act by: a. increasing the activation energy for a reaction b. lowering the activation energy…
A: An enzyme has the ability to attract the substrate to the active site which further results in the…
Q: With the aid of diagrams, describe how the body’s adaptive immune system responds to an infection.
A: Memory cells (B- and T-lymphocytes) are white blood cells that keep track of every germ the immune…
Q: HN-CH- HN-ÇH-C- -OH HN- CH 1 2 3.
A: Each amino acid has a N-terminal (-NH2 group), a C-terminal (-COOH group) & a R-group or the…
Q: Visceral fat loss is often associated with increased insulin sensitivity. Assuming that with the…
A: D. -10.6
Q: Part 1 See Periodic Table O See Hint For the regulation of the purine synthesis pathway shown, what…
A: Given Pathway is for ATP and GTP de novo synthesis from Robose-5-PO4 and pathway shows various level…
Q: True or False? It takes Carbon dioxide and water in the presence of sunlight to produce…
A: Carbohydrates are formed in green plants by the process of photosynthesis.
Q: How is Pyruvate Kinase Deficiency (PKD) inherited? What gene is responsible for the expression of…
A: Pyruvate kinase is an enzyme that regulates cell metabolism by catalysing the conversion of…
Q: The vast majority of structures deposited in the Protein Databank (>95%) have been determined using…
A: “Since you have asked multiple question, we will solve the first question for you. If youwant any…
Q: In your own words, what do you think is/are the reason/s why most of the clinical features of these…
A: Krebs cycle disorders are a set of rare human diseases with a remarkable level of complexity when…
Q: How many FADH2 and NADH molecules get produced by beta oxidation of palmitic acid (a 16 carbon fatty…
A: Beta oxidation is a process of breakdown of fatty acid molecules by breaking the bond between beta…
Q: S. Why are histamine and serotonin contents increased in the site ol inilammatory? Explain the…
A: Histamine is an organic nitrogenous compound which is synthesized from amino acid residue…
Q: PTP1B Substrate kcat kca Km 10-7 x (8-1 м1) DADEPYLIPQQG DADAPYLIPQQG DAAEP YLIPQQG АAААpYLIPQQG…
A: Michaelis-Menten model of enzyme kinetics is a very useful model which describe the relationship…
Q: 4. During a lunch at a MeDonald's outlet, an office employee received about 350 g of carbohydrates…
A: "Since you have asked multiple questions, we will solve the first three subparts of the question for…
Q: Fill in the blanks in the table below regarding the similarities and differences between two…
A: The replication of DNA involves opening up a double-stranded DNA molecule and creating a…
Q: explain why the protein binding of the drug is not correlated with the distribution or elimination…
A: Many drugs form drug macromolecule complexes by interacting with plasma, tissue proteins, or other…
Q: HomeExpert Q&AMy answers How is serine related to the activated methyl cycle? Serine’s side chain…
A: Serine is a non-essential amino acid. It is a glucogenic amino acid. Serine undergoes deamination…
Q: Gold nanoparticles are applied in cancer therapy; approaches such as photothermal therapy as well as…
A: Photothermal therapy is the process of using electro magnetic radiation for treatment of cancer.…
Q: What type of monomers make up proteins? What are protein polymers called?
A: Note - Hi ! Thank you for the question. We are authorized to answer one question at a time. Since…
Q: What is the direction of transcription in the plasmid shown below? Explain your answer based on the…
A: Transcription is the process of synthesis of RNA using DNA as the template and is catalyzed by
Q: Enzymes accelerate the rate of a reaction by: a. lowering the number of molecules with lower…
A: Introduction: Enzymes are biological catalysts that fasten the speed of the chemical reaction. They…
Q: True or False? a. RNA, DNA, and ATP are important macromolecules, are formed from simple…
A: The nucleic acid polymer has nucleotide as its monomeric unit. synthesis of…
Q: n the discovery of new drugs, the chemical and physical properties of a molecule are very important…
A: Hi! Thanks for your question. As you have posted multiple questions and have not mentioned which one…
Q: What enzyme is the point of regulation for fatty acid synthesis in the cytoplasm? Fatty acid…
A: Fatty acids are important micromolecules which combine together to form lipids in plants, animals…
Q: How might a synthetic detergent be superior to a normal soap?
A: INTRODUCTION - Soaps are sodium or potassium fatty acid salts made from hydrolysis of fats in…
Q: Which amino acid(s) are more commonly found at the At which position(s) are amino acids limited to…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: How many molecules of water are produced by the electron transport chain during the complete aerobic…
A: Stearate is saturated fatty acids. It is metabolized by beta oxidation in the cytoplasm. It releases…
Q: Renata enzymatically conjugates a "C-labeled cysteine to a transfer RNA (IRNA), with a UGU…
A: The genetic codon is the three letter codon that specifies the twenty naturally occurring amino…
Q: 1. Proteins act as structural components such as keratin of hair and nail, the collagen of the bone…
A: "Since you have posted a question with sub-parts we will answer the first 3 question for you. If you…
Q: When specific conditions are met, the creation of peptide bonds rather than the hydrolysis of…
A: Proteins are unbranched polymers constructed from 20 standard α-amino acids. They have four levels…
Q: Design a quantitative research experiment to investigate the influence of pH or temperature on the…
A: Introduction: Enzymes are biological catalysts that fasten the speed of the chemical reaction. They…
Q: the 3 major pathways that eventually become entry points of molecules into the Krebs Cycle? What…
A: TCA / Krebs cycle : An ingenious series of reaction catalyzes by eight different enzyme that…
Q: 3. (а) 0.0050 M operate at one-quarter of its maximum rate? At what substrate concentration would an…
A: To study an enzyme kinetics, Michaelis-Menten kinetics equation is best known mathematical…
Q: Succinyl-CoA Synthetase mechanism Succinyl-CoA synthetase enzyme active site O Substrates bind to…
A: Succinyl-CoA-Synthetase: This is an enzyme of TCA cycle catalyze the conversion of Succinyl-CoA to…
Q: Describe the process of DNA replication, including the enzymes involved (85%), and give two examples…
A: DNA replication is a process of duplication of genetic material during the process of cell division…
Q: 8. Which of the following amino acids in hemoglobin accepts proton H+ when red blood cells reach…
A: Hemoglobin is a conjugated protein with four polypeptide chains. It contains two identical alpha and…
Q: Choose the wrong, Release of energy (ATP) comes from the Select one: O a. when the terminal…
A: ATP is the energy currency of the cell.
Q: With Fehling's reagent (under certain conditions) interact: A. Glucose B. Quinine hydrochloride C.…
A: Fehling's reagent is a reagent commonly employed in differentiation of water soluble carbohydrates…
Q: 2. Complete and identify the enzymes involved in the following pathways OydroyacetoreP tructose…
A: The glycolytic pathway helps to metabolize glucose molecules obtained through the diet, glycogen…
Q: What does protein function in the human body?
A: Proteins are made up of amino acids or we can say that amino acids are the building block of…
Q: Which of the following substances ATP, CoA-SH, FAD and NAD+ have the subunits in their structure?…
A: FAD/FADH2, NAD+/NADH, and NADP+/NADPH are cellular electron carriers and ATP is the energy currency…
Q: How many more acetyl CoA are generated from stearic acid than from linoleic acid during beta…
A: The process of beta oxidation of stearic acid yields two distinct products and these are acetyl-CoA…
Q: In Metabolic Pathways, what are the nucleotide-containing compounds?
A: A nucleotide mainly consists of a sugar molecule that is either ribose in RNA or deoxyribose in DNA…
Q: Explain the difference between saturated and unsaturated fats.
A: The chemistry of the triacylglycerols determine their physical properties.
Q: Amino acids with non-polar side chains are zwitterions at a. middle pH levels, between the pKa’s of…
A: Amino acids are compounds with an amino group, a carboxyl group, a hydrogen atom, and a variable…
Q: Make a concept map covering about the following: a. SYPHILIS b. Anti-Streptolysin O Test (ASO TEST)…
A: Hi! Thank you for the question. We are authorized to answer three subparts at a time, since you have…
Q: What is substrate-level phosphorylation? 2. Although oxygen does not participate directly in the…
A: ATP is the energy currency of the cell and is praoduct of catabolic pathways that is used in…
Q: c. (i) Which enzyme in prokaryotes synthesizes the primers? On which strand (leading or lagging…
A: DNA replication, or copying of a cell's DNA, is semiconservative, which means that each strand of…
Q: Basing off of the structures of monosaccharides, explain how a simple sugar can nucleophile. You may…
A: Electrophiles are electron deficient groups. Nucleophiles are electron rich groups.
Q: Indicate a positive result with a (+) sign, and a (-) if otherwise. A triple (+) may be used to…
A: Molisch’s test - is used to detect carbohydrates. Molisch test gives positive for all carbohydrates…
Step by step
Solved in 2 steps
- Below is kinetic data obtained for an enzyme-catalyzed reaction. The enzyme concentration is fixed at 100 nM. Using a Lineweaver-Burke plot, calculate the Vmax value for this reaction. Report your answer to four significant figures in units of uM/min.Which of the following steps should be followed when performing kinetics experiments? the michaelis constant must be known the enzyme and substrate should not be mixed until just before the absorbance readings are taken reaction rates must be taken until reaction completion enzyme concentration must remain constantAn experiment on enzyme-catalyzed reaction was conducted in the laboratory by a student. Results obtained are summarized in the table below. In all the experiments, the concentration of the enzyme is the same. Substrate Concentration Velocity (pmol/min) (pmol) 1.5 0.21 3 0.28 4 0.32 0.36 8 0.4 15 0.45 18 0.47 1. Plot or graph these results using the Lineweaver-Burk method. 2. Determine the KM and Vmax values. Show all equations and calculations.
- An enzymatic reaction follows M-M kinetics with Vmax= 2.5 mol m-3s-1and Km = 5 mM.Calculate the time required for 50% conversion of the substrate in a batch reactor if theinitial substrate concentration is0.2 M.Show your calculation steps.Explain all aspects of enzyme kinetics. You must explain all terms that relateto measuring and comparing enzyme velocity. What are the two enzyme"constants" you would teach to a senior level undergraduate course. Howwould you determine values of these under experimental conditions (i.e. invitro).An experiment on enzyme-catalyzed reaction was conducted in the laboratory by a student. Results obtained are summarized in the table below. In all the experiments, the concentration of the enzyme is the same. Substrate Concentration Velocity (pmol) (pmol/min) 1.5 0.21 0.28 4 0.32 6 0.36 0.4 15 0.45 18 0.47 1. Plot or graph these results using the Lineweaver-Burk method. 2. Determine the Km and Vmax values. Show all equations and calculations.
- Which of the following statements are false? Initial velocities of enzyme reactions are best obtained in the absence of product because it simplifies analysis. Initial velocities refer to the velocity of the reaction right after it is initiated. The velocity of the reaction as a function of measuring time are curved just like an isothermal binding curve because of substrate binding to the enzyme. Initial velocities correspond to the pre-steady state condition for free enzyme. Initial velocities can sometimes be measured by spectroscopy such as UV/Vis spectroscopy when monitoring the production of NADH from NAD+. The velocity of the reaction will eventually go to zero. The reaction will reach equilibrium because of the presence of the enzyme. It is always better to use substrate rather than product to measure enzyme kinetics.Briefly describe why the Michaelis-Menten equilibrium-assumption cannotbe applicable to all enzyme-catalyzed reactionsA technician prepares a buffer solution that will be used to facilitate the optimal pH for an enzyme involved in the biotechnological degradation of organic compounds. The buffer compound will facilitate a stable pH. To prepare the buffer they need to determine the required concentration of anita hara [conjugate acid or base]_08 They have been provided with the buffer parameters provided in the images. Provide the answer to three decimal places and include an appropriate unit. Note: You may need to round the numbers to get the required answer. Final volume of solution: 200 ml VEENER Total buffer compound concentration: 150 mM Ratio conjugate base/weak acid: 1.58/1 2000 Concentration weak acid: 0.058 M Final pH of solution: 6.5 Buffer compound pk.: 6.3 14 13 12 11 10 9
- Regarding the reasoning for the Michaelis-Menten equation to be unsuitable for accurate analysis of experimental data, select all that apply: It is nonlinear It is only valid for reactions at equilibrium It is not valid under experimental conditions Extrapolation to Vm is inaccurate and therefore Km also cannot be accurately describedIf the data from an enzyme experiment is plotted as a Lineweaver-Burk plot, and the Vmax is 0.02 mol/sec, and x-intercept is –2.5 mM then what is the KM value? Show yourwork/reasoning.Below is kinetic data obtained for an enzyme-catalyzed reaction. The enzyme concentration is fixed at 100 nM. Using a Lineweaver-Burke plot, calculate the kcat value for this reaction. Report your answer to three significant figures in units of 1/sec.