Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 8.1, Problem 1MI
MICRO INQUIRY Which types of agents can be used for sterilization? Which can be used for antisepsis? What is the difference?
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1(a) 1. What is the theory upon which intermittent sterilization is based?
(B) Is dry or moist heat more efficient as a sterilizing agent? Why?
(C) When would it be necessary to use aseptic techniques when inoculating a tube of nutrient broth with dirt?
(D)Why do some microorganisms characteristically form a growth of the pellicle type? What environmental factors might alter the formation of a pellicle?
(This is not a graded assignment)
Define the following terms; Sterilization, and Aseptic Technique. What are the
differences between the two concepts?
EXPERIMENT:EFFECT OF CARBON SOURCES ON THE MICROBIAL GROWTH.
1.OBJECTIVE
To study the effect of media components on microbial growth
2.PROCEDURES
Preparation of fungal suspension inoculums
Prepare the laminar cabinet for the inoculum preparation by applying normal aseptic techniques
To a full-grown fungal Petri dish, add 25ml sterilized distilled water and rub the agar gently with glass rod
Pour the suspension into an empty flask and continue previous steps to achieve your desired volume of inoculums suspension.
Cotton-plugged and aluminium-wrapped the mouth of inoculum flask.
For certain fungal species like Niger, the suspension from the agar should be filtered upon pouring into the inoculum’s flask.
Preparation of salt media of different carbon sources.
Prepare the Modified Czapek Dox media by weighing the following salts according to your desired working volume.
KH2PO4 (1 g/L)
NaNO3 (3 g/L)
MgSO4.7H2O (0.5 g/L)
Carbon source (30 g/L)
KCl (0.5 g/L)
2.…
Chapter 8 Solutions
Prescott's Microbiology
Ch. 8.1 - MICRO INQUIRY Which types of agents can be used...Ch. 8.1 - Retrieve, Infer, Apply 1. Define the following...Ch. 8.1 - Retrieve, Infer, Apply 2. What is the difference...Ch. 8.2 - MICRO INQUIRY Examine graph (a). How long would it...Ch. 8.3 - MICRO INQUIRY How might one verify that filtration...Ch. 8.3 - Retrieve, Infer, Apply 1. What are depth filters...Ch. 8.3 - Retrieve, Infer, Apply 2. Describe the operation...Ch. 8.3 - Retrieve, Infer, Apply 3. During the 20092010...Ch. 8.4 - Retrieve, Infer, Apply 1. Describe how an...Ch. 8.4 - Retrieve, Infer, Apply 2. In the past, spoiled...
Ch. 8.4 - Prob. 2.1RIACh. 8.4 - Retrieve, Infer, Apply 2. What is the correlation...Ch. 8.5 - MICRO INQUIRY Why is it important that all of...Ch. 8.5 - MICRO INQUIRY Why are cross-linking agents such as...Ch. 8.5 - Retrieve, Infer, Apply 1. Why are most...Ch. 8.5 - Retrieve, Infer, Apply Construct a table that...Ch. 8.5 - Retrieve, Infer, Apply 3. Which disinfectants or...Ch. 8.5 - Retrieve, Infer, Apply 4. How do phenolic agents...Ch. 8.5 - Retrieve, Infer, Apply 5. Which physical or...Ch. 8.6 - Retrieve, Infer, Apply 1. Briefly explain how the...Ch. 8.6 - Retrieve, Infer, Apply How does being in a biofilm...Ch. 8.6 - Retrieve, Infer, Apply Suppose hospital custodians...Ch. 8.6 - Retrieve, Infer, Apply Briefly describe the phenol...Ch. 8.6 - Retrieve, Infer, Apply Why might it be necessary...Ch. 8.7 - Retrieve, Infer, Apply How would you explain to a...Ch. 8.7 - Retrieve, Infer, Apply Propose the use of specific...Ch. 8 - Throughout history, spices have been used as...Ch. 8 - Design an experiment to determine whether an...Ch. 8 - Suppose you tested the effectiveness of...Ch. 8 - The death of Geobacillus stearothermophilus spores...
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