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You need a digestion buffer consisting of the following: / Jy moet 'n verteringsbuffer op maak wat uit die volgende bestaan: 15m M NaCl 75 mM Tris-Cl,pH 8 16 mM EDTA,pH 8 0.8% sodium sulphate / natrium dodecyl sulfaat 0,75 mg/ml proteïnase K How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations. Remember to explain exactly how you will make it up.

You need a digestion buffer consisting of the following: / Jy moet 'n verteringsbuffer op maak wat uit die volgende bestaan: 15m M NaCl 75 mM Tris-Cl,pH 8 16 mM EDTA,pH 8 0.8% sodium sulphate / natrium dodecyl sulfaat 0,75 mg/ml proteïnase K How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations. Remember to explain exactly how you will make it up.

Principles of Modern Chemistry
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter17: Electrochemistry
Section: Chapter Questions
Problem 80AP
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You are supplied with the following: / Jy word voorsien van die volgende:

  • NaCl(Mr= 58,443 g /mol)
  • 2.5MTris-Cl, pH 8 solution /oplossing (1 Litre)
  • EDTA,natriumsout(Mr= 380,2g/mol)
  • 10% sodium dodecyl sulphate solution / natriumdodecyl sulfaat oplossing
  • Proteïnase K solution / oplossing (50 mg dissolved / opgelos in 1 ml ddH2O)

You need a digestion buffer consisting of the following: / Jy moet 'n verteringsbuffer op maak wat uit die volgende bestaan:

  • 15m M NaCl
  • 75 mM Tris-Cl,pH 8
  • 16 mM EDTA,pH 8
  • 0.8% sodium sulphate / natrium dodecyl sulfaat
  • 0,75 mg/ml proteïnase K

How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations. Remember to explain exactly how you will make it up.

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