You are trying to determine, which if any of the children of Brenda and Ike are actually fathered by Ike. You analyze 2 genes known to contain variable numbers of repeats by PCR and Gel Electrophoresis and get the following results Ike Brenda Joe Tina Holly Ralph Ike Brenda Joe Tina Holly Ralph Based on this information a) Joe must be the child of Ike and Brenda b) Joe could be the child of Ike and Brenda c) Joe can not be the child of Ike and Brenda Gene 1 Gene 2
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Genetics Question 8
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- The results of a paternity test using short tandem repeatsare listed in the table below. Who’s the daddy? How sureare you?After doing PCR in several labs, 29 homozygous large, 62 heterozygotes, and 9 homozygous small bands were obtained. What is the frequency of the small alleleThe picture shows a gel of PCR products for the loci SRY, AMELX/Y and ZFX/Y, for three controls and five test human individuals (1-5). Below the gel is the phenotypic sex of each individual, their fertility status, and the number of Barr bodies seen in the nucleus of a somatic cell in each case. The diagram to the right shows the positions of the tested loci on the human sex chromosomes, and also the positions of the PRKX and PRKYgenes. Which sample carries a derived X chromosome with a translocation due to ectopic crossover between the loci PRKX and PRKY: 46,X,derX,t(X,Y); ? 1 2 3 4 5
- The diagram below represents results of agarose gel electrophoresis performed after PCR amplification of a molecular marker in diploid organisms. Answer the following questions: a) How many individuals are homozygous and how many are heterozygous? b) How many alleles are there in this population?Three newborn bables have been separated from their parents. You need to make sure that each child is reunited with the proper set of parents. The data below was gathered by amplifying two VNTR loci in each baby and the possible parents. Fragments were then separated using gel electrophoresis. Assume that each parent couple had only one of the babies. Match the babies with the proper parents. VNTR Locus 1 Baby 1 Baby 2 Baby 3 Jeff Jane David Dianna Mike Maddle VNTR Locus 2 Baby 1 Baby 2 Baby 3 Jeff Jane David Dianna Mike Maddie Baby y Choose... Mike and Maddie Baby David and Dianna Jeff and Jane BabyThe following information was obtained during our Weeks 8 and 9 labs: PCR stands for a polymerase chain reaction. 5 students had homozygous positive (+/+) genotypes, 8 students had heterozygous (+/-) genotypes, and 21 students had homozygous negative (-/-) genotypes, out of the 34 effective results. To calculate the amount of positive and negative alleles in this gene pool, add them together. Fill in the Hardy-Weinberg equation with these numbers. Is Bio 30's population close to Hardy-Weinberg equilibrium? Demonstrate your equations and reasoning.
- Consider the first category of test-cross offspring shown in figure 8.2 (+b, LS). Consider also that the parents of the heterozygous female flies in the test cross had the following genotypes: bb, SS, and +, LL. A. What would be the physical phenotype of these flies? B. If PCR was conducted with the DNA of one of these flies using the primers for the molecular marker, what would be the appearance of the bands on an electrophoresis gel with the PCR products? C. If the gene for black body and the locus for the molecular marker (L long or S short) were unlinked, what proportion of the test-cross progeny would be black flies that are heterozygous for the molecular marker? What proportion would be flies with normal body color, which are homozygous for one form of the molecular marker? D. If the gene for black body and the locus for the molecular marker were linked, how would the proportion of flies be different?A blood stain from a crime scene and blood samples from four suspects were analyzed by PCR using fluorescent primers associated with three STR loci: D3S1358, vWA, and FGA. The resulting electrophoretograms are shown below. The numbers beneath each peak identify the allele (upper box) and the height of the peak in relative fluorescence units (lower box). Solve, (a) Since everyone has two copies of each chromosome and therefore, two alleles of each gene, what accounts for the appearance ofonly one allele at some loci? (b) Which suspect is a possible source of the blood? (c) Could the suspect be identifi ed using just one of the three STR loci? (d) What can you conclude about the amount of DNA obtained from Suspect 1 compared to Suspect 4?A female cheetah recently gave birth while in captivity; however, the paternity of the infant is unclear. Scientists tested the DNA from two male cheetahs, along with the female and offspring. The resulting electrophoresis gel is shown. Based on the gel electrophoresis results, what can be concluded about the paternity of the offspring?
- Based on the table below, what is the order of the genes examined? a b с d A B C/C B A Selected answer will be automatically saved. For keyboard navigation, press up/down arrow keys to select an answer. A C B/B C A CAB/BAC Phenotype of testcross ССА/АСС A,C C A, B, C A B, C A, B B Genotype of hybrid gamete Abc ab C A B C Abc a B C abc е Not enough information A B C a B c # of progeny 388 5 50 64 51 47 5 390 1000Using the data below please identify the following: Identify which of the siblings in generation II has a recombinant chromosome. Determine which of his/her children is at risk of developing adenomatous polyposis based on the new data. Table 2 RFLP data for a family suffering from APC syndrome. III1 III2 III3 II1 II2 III4 III5 III6 I1 I2 II3 II4 III7 II5 II6 III8 III9 III10 II7 II8 III11 III12 III13 II9 II10 A A A A A A A A A A A A B B B B B B B B B B B B B B B B B B B C C C C C C C C D D D D D D DUsing the pedigree chart, explain: a) The number of generations seen. b) If all blue-coloured shapes are affected with disease X- how many males are affected? how many females are affected? c) Does this disease have a dominant or recessive inheritance pattern? Justify your answer.