You are examining a blood smear and see microbial cocci in the smear. You know a leukocyte/white blood cell diameter is approximatley 9 micrometers. You see 3 microbes, side by side, span the diameter of the leukocyte. What is the diameter of 1 microbe including correct units?
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- We will be using a variety of media/tests to identify the unknown organisms. In the table below identify what each test can tell us about our unknown organisms. Media or Test What it tells us? Positive Result(s) Negative Result(s) Additional Information (e.g. reagents used, etc.) Blood agar plate Exotoxin Hemolysins produced, are able to destroy red blood cell and hemoglobin -Complete hemolysis, produce clearing around: Beta hemolysis -Incomplete hemolysis, produce greenish discoloration around colonies- Alpha hemolysis No breakdown – Gamma hemolysis Catalase test Citrate slant Gram stain Decarboxylase broth: Lysine Decarboxylase broth: Ornithine DrySlide (Oxidase Test) Hektoen Enteric Agar Mannitol salt agar Milk Agar Motility test MRVP broth: MR MRVP broth: VP Nutrient gelatin tubes Nitrate…How many different types of colonies can you find on the blood agar plate? describe each. (As shown in pictures below) What possible types of bacteria ? (As shown in pictures below)Vida works in the haematology laboratory of a smallhospital. The laboratory’s haematology procedure manualcontains instructions for staining blood smears and callsfor applying the stain for 2 minutes and the buffer for 2minutes. Vida stained three patient blood smears, but thesmears appeared bluer than normal. When she examinedthe smears microscopically, the cells also appeared blue,making it difficult to identify them.1.What can cause blood smears to stain too blue?2.Discuss factors that could have affected the stain results.
- The physician ordered erythromycin 500 mg IV every 8 hours for infection. The pharmacy sends a vial labeled: Erythromycin 1 g. The nursing drug reference provides information to reconstitute 1 g of erythromycin with 20 mL of sterile water and further dilute in 250 mL of 0.9% NS and to infuse over 2 hours. How many ml will you draw from the vial after reconstitution and add it to the IV bag of 0.9% NS 250 mL? 20 mL 5 mL 15 mL 10 mL Page 10 of 14 Previous Page Next PageA cell count was performed on a serous fluid specimen. As the fluid was cloudy, a 1:5 dilution was prepared. The counts were: 34 and 32; 5 squares on each side were counted. What is the average cell count per L? Side 1: 34 x 1 x (1/(5x0.1)) x 5 = 340 per µl Side 2: 32 x 1 x (1/(5x0.1)) x 5= 320 per µlHelping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.
- The predominance of squamous epithelial cells with few WBCs in a sputum sample would be adequate for diagnosis and culture. true falseThis plate shows: Beta hemolysis Alpha Hemolysis Delta hemolysis Gamma hemolysisYou are given a sputum sample to make serial dilutions on a Friday. The pathologist tells you that the patient is very sick so bacteria should be very concentrated you will need to dilute it (assume that the sample is concentrated at ~1x107 CFUs/mL). But you only have 2 sterile tubes to make dilutions! It is Friday and you want to go home because you are tired and/or want to go have fun with your friends. Since you don’t have time or don’t want to clean and sterilize more tubes to make more dilutions, you decide to make do with the two tubes you have available. How would you dilute your sputum sample to get a countable plate by using only two tubes for dilutions? You have to make sure you get a countable plate. You have to explain your dilutions in detail, this means you need to specify not only dilution factors but the volumes you are using to get to those dilutions.
- This microbe is resistant to the following anti microbials? Which apply? VA AMC LZ CTR PA patient 12 in. thick is positioned on a table 15 in. from the target of an x-ray tube. The region of interest in the patient is 6 in. in diameter and 3 in. above the table. Can the entire region be displayed at once with an image intensifier with an input screen 9 in. in diameter?On your own words, identify the different problems associated with routine blood smear preparation