Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…
A: The Lac operon contains genes responsible for the transportation and breakdown of lactose. These…
Q: What role does the I gene product play in regulation of the Lac operon
A: The operon plays an important role in bacteria. The lac operon is an essential operon useful for…
Q: If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not…
A: Operon acts as a single regulated unit having one or more structural genes, promoter gene, a…
Q: How does catabolite repression work on gal operon?How could you decide if one particular operon is…
A: catabolite repression : Catabolite repression is a regulatory system by which any microorganism…
Q: Would a mutation that inactivated lac repressor and prevented it from binding to the lac operator…
A: The negative regulation of the lac operon is done by a repressor, which is a product of the lacI…
Q: How does repression work in the lac operon?
A: Lac operon consists of a promoter, operator group of structural genes such as lac A, lac Z, and lac…
Q: Please describe in full detail how the LAC operon is controlled by both negative gene regulation and…
A: Lac operon is a gene controlling nit which contains operator, promotor and structural gene region.…
Q: A mutation at the operator prevents the regulator protein from binding. What effect will this…
A: An operon includes a group of genes that have a common promoter, regulator, and would be transcribes…
Q: Operon X produces three enzymes that convert Compound A to Compound B. The R gene makes the…
A: Gene expression is given on the basis of RNA. It is a process through which there is turning on of…
Q: What do you mean by coordinate expression of genes in operon?
A: Answer- In prokaryotes genes are controlled by one promoter region that is called polycistronic…
Q: Regulation of the E. coli trp operon by attenuation requires the trp repressor protein. prevents…
A: An operon is a function unit of DNA containing a set of genes including structural gene, a…
Q: Compare and contrast the functional mechanism of the Inducible - Lac operon with the Repressible -…
A: Gene expression is a process in which the genetic instructions of genes are utilized to manage…
Q: The arabinose operon How does the araC protein regulate of the arabinose operon. How does its…
A: How arac protein regulate arabinose operon and how binding differ If arabinose is absent, the dimer…
Q: A bacterial cell is mutated and cannot create the repressor protein associated with the Lac operon.…
A: Lac operon is associated with uptake and metabolism of lactose. It has several components such as a…
Q: Which of the following arabinose operon regulatory sites is exclusively involved in the repression…
A: Hi, Thanks For Your Question. Answer : Correct Option Is Ara O2.
Q: Let’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively…
A: E.coli is a gram negative bacterium which thrives in the intestine of mammals. E.coli is beneficial…
Q: If an abnormal repressor protein could still bind allolactose, but thebinding of allolactose did not…
A: Deoxyribonucleic acid is a particle made out of two polynucleotide chains that loop around one…
Q: In order for the lac operon to be triggered, lactose must be present and ________. a. cAMP levels…
A:
Q: If there was a mutation such that the lac I protein could no longer bind lactose, but could still…
A: Lac operon is responsible for the metabolism of lactose in bacteria.
Q: 1d regulation of the lack operon by the lack repressor is an example of negative regulation. what in…
A: E.coli bacterium intake glucose as a primary source of energy metabolism . When a glucose is scarce…
Q: You are growing E. coli in a laboratory in order to study their operons. The growth media you are…
A: 1. What effect does lactose have on the bacterial cell’s lac operon? Answer:- It induces the operon,…
Q: Explain how the following conditions will affect the regulation of the operon. Low cAMP levels; E.…
A: Operon is a sequence of DNA that consists of a set of genes which are involved in a particular…
Q: Which of these operons is most similar to the lac operon? Explain.
A: A group of genes that transcribe to produce one mRNA is called an operon. This operon is responsible…
Q: How do we know that the trp operon is a repressible controlsystem, in contrast to the lac operon,…
A: Introduction The process by which the information contained in a gene is used to drive the assembly…
Q: What led researchers to conclude that a repressor moleculeregulates the lac operon?
A: Lac operon is regulated by the lac repressor molecule.
Q: Give all possible genotypes of a lac operon that produces, or fails to produce, β-galactosidase and…
A: An operon is defined as a group of genes that have a common promoter and regulator and also…
Q: I need help with answer 2 and 3
A: Ans. 2 promoter sequence
Q: What is the difference between positive regulation of the lac operon and negative regulation of the…
A: The lac operon is a set of three bacterial genes under the control of a single promoter which is…
Q: One way that regulation is often studied is by examining the effects of mute ons. For example,…
A: There are two types of lac mutations occurring in lac operon: 1. uninducible - operon can't be…
Q: Bacterial DNA containing an operon encoding three enzymes is introduced into chromosomal DNA in…
A: Prokaryotes are the organisms that lack the cell nucleus and membrane-bound organelles. They have…
Q: A mutation at the operator prevents the regulator protein from bindingWhat effect will this mutation…
A: A group of genes that are transcribed under a single promoter and an operator is referred to as…
Q: Lac operon experiment,
A: In bacteria, the operon is the coordinated unit of genetic expression and is the functioning unit of…
Q: Can you think for how long the lack operon would be expreesed in the presence of lactose?
A: Lac operon is an operon that regulates the breakdown of lactose present in the medium. It contains…
Q: Assuming that the level of glucose is low, a mutation in the repressor associated with the lac…
A: The lac operon which is also known as lactose operon is a set of genes that are specific for uptake…
Q: High levels of glucose in the environment result in low levels of transcription of the lac operon.…
A: The lactose operon (lac operon) is an operon needed for the transport and digestion of lactose in…
Q: For some time it was not clear whether lac repressor inhibits lac operon transcription by inhibiting…
A: Lac operon is a transcriptional regulation of lactose metabolism seen in prokaryotes. It was…
Q: What is a specific region in the trp operon that you could mutate so it expresses the trp operon in…
A: Introduction : The Trp Operon Codes For The Components Required For Tryptophan Production. An Operon…
Q: Describe in details all the differences between The Trp Operon and the Lac Operon. Why one is always…
A: As operon is that the cluster of genes expressed along from single promoter and lac and trp area…
Q: In the medium where E.coli was growing, lactose was added, which induced the lac operon. Then, w hy…
A: The lac operon is a set of genes that share a single promoter (transcribed as a single mRNA). The…
Q: With respect to the lac operon, what is allolactose?
A: It is an operon required for the transport and metabolism of lactose in E.coli and many enteric…
Q: A mutant strain of E. coli produces β-galactosidase in both the presence and the absence of lactose.…
A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…
Q: Explain why inactivation of the O1 sequence of the lac operator almost completely abolishes…
A: Lac operon of the bacteria includes the structural genes that code for the enzymes involved in the…
Q: The promoter of an operon is the site to which RNA polymerase binds to begin transcription. Certain…
A: Promoter is a binding site for the RNA polymerase in the gene. The RNA polymerase is involved in the…
Q: E. coli is given lactose as a nutrient source. This results in the activation of the lac operon only…
A: The solution to the above mentioned questions is as follows:
Q: In the: Mutation of the regulatory region for a repressor protein in the lac operon. Explain: (a)…
A: Operon It is a set of multiple gene that is controlled by single operator.
Q: Why do scientists define the trp operon as repressible and the lac operon as inducible?
A: Operon is a combination of genes that are transcribed by the same promoter.
Q: If glucose is not available, but lactose is available from the environment, what is the status of…
A: Points to keep in mind related to Lac operon : 1. Presence of Lactose inhibits repressor protein…
Q: If you made a mutation in the operator region of the lac promoter such that LacI can no longer bind…
A: Lac operon Lac operon or lactose operon is a negatively regulated operon containing operator gene,…
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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- If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?Suppose you are studying the regulation of a gene involved in the metabolism of two nutrients, Llamasin and Alpacalon, in bacteria. You are trying to determine if these nutrients act as inducers in their operons. The following data were collected from your experiments. Which of these operons is most similar to the lac operon? Explain. Nutrient Levels of nutrient in growth medium Level of transcription of genes in operon Llamasin low high high low Alpacalon low low high high Highlight one in green: Llamasin Alpacalon Explanation:To study the lac operon, you engineer a strain of E coli to have a lac operon in which the lac Z gene is replaced by the gene for green fluorescent protein (GFP). Expression of GFP generates a green color in the cells that can be easily quantitated with a fluorescence microscope. You test the activity of the operon in the absence of the inducer IPTG, the presence of the inducer IPTG and the presence of an antibiotic the completely inhibits RNA polymerase (i.e. no gene expression). You then use this system to test the effects of various mutation on the activity of the operon. Match the following mutations with the activity (A, B or C) you would expect to observe with the mutation. All experiments are done in the presence of IPTG unless otherwise stated.
- If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?One way that regulation is often studied is by examining the effects of mutations. For example, mutations within the parts of the lac operon have been well studied. Which of these mutants would be considered a constitutive mutant (the operon is always on)? a mutation in CAP that prevents the binding of CAMP a mutation in one of the structural genes that prevents the transport of lactose into the cell a mutation that disables the promoter for the operon a mutation that disables the repressorIn most experiments using the lac operon, researchers use the synthetic inducer IPTG to turn on operon expression, instead of lactose or allolactose. What do you think is the advantage of using IPTG?
- You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, Please explain so I can understand in the answer What effect does lactose have on the bacterial cell’s lac operon? What does the absence of glucose do to the bacterial cell? You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? What kind of regulation does lactose provide to an operon’s repressor?Under what conditions would you expect to see high levels of expression of the structural genes in the lac operon? Group of answer choices Lactose is absent and glucose is absent Lactose is absent and glucose is present Lactose is present and glucose is present None of these Lactose is present and glucose is absentWhich of the following lac operon genotypes would allow for functional versions of all the structural enzymes of the lac operon to be expressed constitutively even in the absence of lactose? Group of answer choices I+ O+ Z+ Y+ A+ I- O+ Z- Y- A- I+ OC Z+ Y+ A+ IS O+ Z+ Y+ A+ I+ O+ Z- Y+ A+
- Describe in details all the differences between The Trp Operon and the Lac Operon. Why one is always off while the other is always ON? Can you find examples for other catabolic and anabolic pathways that could behave in the same way?A theoretical operon (theo) in E. coli contains several structural genes encoding enzymes that are involved sequentially in the biosynthesis of an amino acid. Unlike the lac operon, in which the repressor gene is separate from the operon, the gene encoding the regulator molecule is contained within the theo operon. When the end product (the amino acid) is present, it combines with the regulator molecule, and this complex binds to the operator, repressing the operon. In the absence of the amino acid, the regulatory molecule fails to bind to the operator, and transcription proceeds. Characterize this operon, then consider the following mutations, as well as the situation in which the wild-type gene is present along with the mutantgene in partially diploid cells (F¿):(a) Mutation in the operator region.(b) Mutation in the promoter region.(c) Mutation in the regulator gene.In each case, will the operon be active or inactive in transcription, assuming that the mutation affects the…What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)