Which of the following statements is true regarding DNA replication? Select all true statements. DA DNA polymerase moves along the template strand in the 3' to 5' direction. OB DNA polymerase requires a primer to initiate replication. DC. DNA polymerase forms a covalent bond between a phosphate group and a hydroxyl group OD DNA replication on lagging and leading strand occurs in the 5' to 3' direction.
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- Match the statement to the corresponding agent/key player in DNA replication. Some items require more than one answer. choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable Start of replication in eukaryotes Start of replication in prokaryotes Removal of eukaryotic primer Origin of replication in eukaryotes Adds more nucleotides in the lagging strand of eukaryotes Origin of replication in prokaryotes Adds more nucleotides in the leading strand of eukaryotes Lessens the tension of supercoiling Adds more nucleotides in the leading strand of prokaryotes Dissociates after adding the few initial nucleotides in prokaryotes Holds the processive enzyme in eukaryotes Breaks the H-bonds of bases Adds more nucleotides in the lagging strand of prokaryotes Proof reading in…Place the following steps of DNA replication in order (from left to right) from the beginning to the end of the process. Reset Help priming of DNA synthesis with RNA primer removal of primers by DNA polymerase I exonuclease elongation of RNA primers by DNA polymerase III opening and stabilizing the DNA strands joining of Okazaki fragments by DNA ligase Beginning of replication End of replicationMatch the statement to the corresponding agent/key player in DNA replication. Some items require more than one answer. choices: Origin of replication Bubble SSBP RPA Sliding Clamp PCNA DNA Pol III Pol ε Pol δ DNA Pol I RNAse H Flap 1 DNA gyrase Pol α DNA helicase Primase Single chromosome Multiple points in chromosomes DNA ligase Not applicable Origin of replication in eukaryotes Adds more nucleotides in the lagging strand of eukaryotes Origin of replication in prokaryotes Adds more nucleotides in the leading strand of eukaryotes Lessens the tension of supercoiling Adds more nucleotides in the leading strand of prokaryotes Dissociates after adding the few initial nucleotides in prokaryotes Holds the processive enzyme in eukaryotes Breaks the H-bonds of bases Adds more nucleotides in the lagging strand of prokaryotes
- DNA polymerases are processive, which means that they remain tightly associated with the template strand while moving rapidly and adding nucleotides to the growing daughter stand. Which piece of the replication machinery accounts for this characteristic? Helicase Sliding Clamp Single Stranded Binding Protein PrimaseWhat proteins are crucial for creating and maintaining DNA replication forks? Choose the best explanation. Question 2 options: Helicase creates the replication fork; primase keeps the single strands from closing shut. Helicase creates the replication fork; single-strand binding proteins keep the single strands from reuniting. Ligase creates the replication fork; DNA polymerase II keeps the single strands from reuniting. Helicase creates the replication fork; ligase keeps the single strands from closing shut.Semi-discontinuous replication means that: DNA synthesis only occurs in the same direction as the replication fork. the double helix unwinds and each strand serves as a template for a new DNA molecule. there are Okazaki fragments associated with the leading strand. DNA synthesis occurs in both a 5’ to 3’ and a 3’ to 5’ direction. there is both a leading and a lagging strand associated with each replication fork.
- Show the replication strands in each of these bubbles (note they have different DNA orientations). Label each end of each DNA strand and include arrows to show which direction it is extending. Show the Okazaki fragments in the correct places. 3' 5'Match the following descriptions with the enzymes involved in DNA replication. 1. Adds an RNA primer to begin elongation 2. Removes the RNA primer from the beginning of the newly constructed strands 3. Splices lagging strand segments 4. Cleaves the rung of the DNA double helix ladder Description: DNA DNA Helicase Primase Enzyme: Polymerase LigaseDuring DNA replication, the function of RNA primers is to Group of answer choices serve as a binding site for DNA ligase separate the two strands of the double helix to open replication "bubbles" serve as starting points for DNA strand elongation by DNA polymerase in the 3' - 5' direction prevent new-separated strands of DNA from rejoining serve as starting points for DNA strand elongation in the 5' - 3' direction by DNA polymerase
- Which of the following statements about DNA replication is correct? Group of answer choices the leading strand is replicated continuously, while the lagging strand is replicated discontinuously on the leading strand, the new nucleotides are added only to the 5' end of the nucleotide chain on the lagging strand, the new nucleotides are added only to the 5' end of the nucleotide chain DNA polymerase works from 3'--> 5' onlyWhich of the following most correctly describes a process that occurs during DNA replication? Group of answer choices Replication of the lagging strand occurs in the 5' → 3' direction—the leading strand in the 3' → 5' direction. Replication is continuous on the lagging strand and discontinuous on the leading strand. Okazaki fragments are DNA fragments synthesized on the lagging strand. DNA polymerase adds dNTP monomers in the 3′–5′ direction. Replication is continuous on the lagging strand and discontinuous on the leading strand.Indicate the proteins involved in the following steps of DNA replication in E. coli a. ___________ initiator of replication by binding to the origin of replication of the prokaryote ___________ a dimer that unzips the DNA helix ___________ an enzyme responsible for relieving positive supercoils ahead of replication fork ___________ maintains DNA in single-stranded state ___________ primary replicating enzyme ___________ synthesizes RNA primers The resulting gap by removal of RNA primer is filled in by ___________. h. the resulting nick is sealed by _____________.