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Which medium is well-indicated to amplify a small population of bacteria mixed with much larger bacterial colonies?
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- Given a log phase bacterial culture with 1 x 10^6 cells per ml and a generation time of 30 minutes how long does it take the culture to reach a density of 6.4 x 10^7 cells per ml?What is the similarities of differential centrifugation and gradient centrifugation?How long does it take for a bacterial culture with a generation time of 12 minutes to increase from 400 cells up to 1600 cells/mL?
- Why is bacterial culture useful to grow bacteria?Why should bacterial culture plates be incubated inverted (bottom up)?Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?
- You've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture. Which technique(s) is/are the BEST to utilize? How will the procedure be carried out? Explain.Does agar concentration affect the growth and morphology of bacterial colonies? What causes dense growth and what causes minimal growth?Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours? At the end of 7 hours? Show your solution.
- Why are complex media preferable to chemically defined media for routinecultivation of microorganisms?In the experiment by Bernard Davis, bacterial F+cells and F- cells were growing while separated by a filter. Filter pores allowed the passage of the liquid medium but not the bacteria cells. As a result: 1) prototype colonies grew well on minimal medium 2) F+ cells were converted to F- cells despite the physical separation 3)F- cells were converted F+ cells despite the physical separation 4)F+ cells were not converted to F- cells because of the physical separation 5) F- cells were not converted to F+ cells because of the physical separation 6)there was no growth of prototypes on minimal mediumWhat general type of growth medium would you use to: (a) grow one type of bacteria but inhibit the growth of another type? (b) discriminate between different types of bacteria?