Q: What is a mixed culture? A contaminated culture?
A: A microbiological culture, or microbial culture, is a technique for increasing microbial creatures…
Q: Why is a block of agar inoculated rather than streaking the fungal culture on a plate?
A: The culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth…
Q: Why do you have to boil the agar solution before dispensing it into tubes?
A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…
Q: In spread plate method, colonies form within the agar and agar surface. In pour plate method,…
A: Introduction Agar media is essential for the study of microorganisms and molecular biology, It is a…
Q: Why is mannitol salt agar (MSA) considered both a selective and differential agar for staphylococci?
A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…
Q: You will only isolate pure colony to inoculate again (sub-culturing) to newly prepared plate medium,…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Gram stain of Nodule Suspension Describe the morphology of the crushed nodules under microscopes…
A: The bacteria are stained with the gram stain and can be divided into two groups on the basis of the…
Q: When can gelatin be used in culture media preparation? [Answer in not more than 2 sentences
A: GELATIN: Gelatin, often known as gelatine, is a transparent, colourless, and flavourless culinary…
Q: Why is the membrane filter method preferred to the most probable numbermethod (MPN) for the…
A: Most probable number (MPN) is a method used to estimate the numbers of bacterial cells in a water…
Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…
A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…
Q: How is the CTSA (Coconut tryptic soy agar) growth medium designed to meet the nutritional need of…
A: Coconut trypic soy agar media are general-purpose, non-selective media that provide enough nutrients…
Q: Why is glucose not the choice of carbohydrate for most differential media used in the identification…
A: Bacilli present in the intestinal tract are rod shaped bacteria.for example, enteric bacteria…
Q: A. niger and B. cereus cultures used in this experiment containedspores. Why is B. cereus more heat…
A: Both Aspergillus niger and Bacillus cereus have spores of which the spores of B.cereus are more…
Q: Explain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay…
A: Hematocrit test is used to check the proportion of red blood corposcles (RBC) present. If the RBCs…
Q: attern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface…
A: Slant culture : A culture made on the slanting surface of a solidified medium in a test tube that…
Q: Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition.…
A: A growth medium is a also known as a culture medium which can be solid, liquid, and even…
Q: Name the source from where agar-agar is obtained.
A: Agar-agar is a gelatinous substance that is formed by a combination of linear polysaccharide agarose…
Q: How does McConkey agar serve as both selective medium and differential medium ?
A: MacConkey agar is a culture media that contain bile salts, crystal violet dye, neutral red dye along…
Q: Why after a certain period the growth rate of the culture decreases until growth stops?
A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…
Q: What is culture media? Which culture media is used for gram negative bacteria and which one for gram…
A: Microbiology is the branch of science which deals with the study of different types microorganisms…
Q: What is the laboratory's policy in decontaminating the plates? Since the growth of H. pylori was…
A: Routine decontamination of lab countertops or paintings areas needs to occur frequently. Lab benches…
Q: Is the Peptone Iron Agar (PIA) a complex or defined medium? Explain based on its composition. Is the…
A: Depending on their composition, media can be classified as defined and undefined media. Defined…
Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…
A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…
Q: How does alkaline treatment improve the quality of agar/carrageenan extracted from the seaweed…
A: Agar is a polysaccharide created from two essential additives. Agarose and agaropectin. Agarose is…
Q: why macconkey agar and mannitol salt are regard as salective and deffrental medium?
A: A culture medium is just water and nutrients that support microbic growth. Primary culture media for…
Q: Using the Table above as a guide, indicating what media could be used to differentiate the…
A: Introduction : The bacterium Staphylococcus epidermidis is gram-positive. It is a natural component…
Q: Oven sterilization of aqueous culture medium. Effect: Rationale:
A: Oven sterilization utilizes dry heat of about 160 or 170 degrees celsius for one hour or 30 minutes,…
Q: Why is agar ideal for microbial cultivation?
A: The microbial cultivation is the process in which microorganisms are cultivated in the laboratory,…
Q: What is a pure culture and how can one be obtained
A: Cells are the structural and functional units of life.
Q: What is differential medium
A: Differential medium or indicative media contain compounds which support the growth of microbes and…
Q: WHAT IS THE TYPE OF Growth patterns on Agar Slant and Broth Medium
A: Growth and morphogenesis of plant is determined by the composition of culture media. There are few…
Q: What is the media used in Urease Test and expected results?
A: Urease test is defined as a biochemical test that distinguishes the basic alkaline fermentation of…
Q: if straph are only suppose to grow on the plate what could possibly be wrong with the media ?
A: Staphylococcus aureus is a Gram-positive bacterium which is cocci shaped. It is a facultative…
Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…
A: These are biochemical test is to perform to identify different type of bacteria.
Q: What process would explain why the dyes spread through the agar?
A: Agar -- Agar is polymer and mixture of polysaccharides obtained from the cell wall of red algae .…
Q: Is the Mueller-Hinton Agar (MHA) a complex or defined medium? Explain based on its composition. Is…
A: Mueller and Hinton in 1941 developed Mueller Hinton Agar(MHA). MHA is used for routine…
Q: Can culture media be sterilized in the hot air oven? why or why not?
A: Sterilization is the process of deactivating, killing, or removing any kind of microorganism (or…
Q: Use of streak plate technique to isolate and purify psychrotrophic spoilage-causing bacteria
A: There are billions of bacteria in the human body that make up the natural flora to resist the…
Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…
A: Bacteria are of many types. They can be classified based on different properties such as cell wall…
Q: Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking.…
A: Streaking method This method is used to produce isolated colonies of bacteria. This helps in…
Q: Based on the growth you saw on your Lee’s Agar plate, what conclusions can you make about the…
A: Lee Agar is used to study the bacteria that can grow on yogurt. It is primarily used to count the…
Q: What type of agar is MacConkey agar?
A: MacConkey agar or MAC which is also described as a bacterial culture medium was named after the…
Q: What is agar and what makes it the best agent for making culture media? explain
A: Microbiology is the branch of science which deals the study of different microorganisms such as…
Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: Assume that you had plated your +PGLO culture on an LB agar plate. What result (number of colonies)…
A: +pGLO culture is a type of bacteria that has been genetically modified to produce a fluorescent…
Q: explain why you would expect to see similarities in the types of colonies on the fomite plates and…
A: Organisms that are too small to be seen by the naked eye are microorganisms. These organisms can be…
Q: Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different…
A: The liquid or solid substratum on which, the organ or cell explants can be grown. The medium…
Q: What is the preparation condition for culturing a mould sample?
A: Introduction: Mould is a fungal specimen which is grown in filamentous structure called hyphae. They…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
Which culturing method provided the clearest results, the semisolid medium tube or the soft agar plate?
Explain.
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- On agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why can a single colony on a plate be used to start a pure culture?Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?What is agar and what makes it the best agent for making culture media? explain
- Colonies have grown in a nutrient agar plate. However, it did not grow along the lines of streaking. Is this normal? If not, what could be the problem?What is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?What is the preparation condition for culturing a mould sample?
- Name the source from where agar is commercially obtained.Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to growIdentify the texture, transparency, color, colony size, form, margin and elevation of the two colonies in the photo (a) and (b)