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- A stock solution was diluted in two steps. In a first step, 0.5 ml was diluted with 9.6 mL buffer. In a second step, 0.5 mL from the first step was diluted with 9.6 mL of buffer. The concentration of the mixture of the second step was determined to be 0.24 mg/mL. What was the concentration of the stock solution?What is the function of buffer systems in living organisms and why are they important in the chemistry of the circulatory system?what is the molecular nature of the substance responsible for the acid-fast property of an organism?