Wheeler Johnson Principle: Reagents: Reaction: Substance Detected: Murexide Principle: Reagents: Reaction: Substance Detected: 10% NH4OH & 2% AgNO3 Principle: Reagents: Reaction: Substance Detected:
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Wheeler Johnson
Principle:
Reagents:
Reaction:
Substance Detected:
Murexide
Principle:
Reagents:
Reaction:
Substance Detected:
10% NH4OH & 2% AgNO3
Principle:
Reagents:
Reaction:
Substance Detected:
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Solved in 2 steps
- Explain the chemical basis (reaction) of Millon’s test. (Generic reaction and explanation) What is the difference between Millon's test and Biuret test?(minimum 5)Using DEAE-cellulose as ion exhange resin, indicate the starting and ending pH for the narrowest experimental pH range used to seperate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: Ending pH:Calcium phosphate is used as an antacid, supplement, and/or a source of calcium and phosphate in toothpaste. In a laboratory setup calcium hydroxide can be reacted with phosphoric acid to produce calcium phosphate with water as by- product. In an experimental process, there are three procedures to deal with in the production of calcium phosphate. Procedure 1: 846.0 grams of calcium oxide and 264.0 grams of water are combined in order to produce a single product, calcium hydroxide. Procedure 2: 979.0 grams of diphosphorus pentoxide and 323.0 grams of water are combined in order to produce a single product, phosphoric acid. Procedure 3: Actual mass of isolated calcium hydroxide from Procedure 1 and actual mass of isolated phosphoric acid from Procedure 2 are combined in order to produce calcium phosphate with water as by-product. It is also noted that each procedure had the following percent yields: Procedure 1: 53.58% yield on calcium hydroxide. Procedure 2: 59.26% yield on phosphoric…
- Bradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NAOH. Store at 4°C until precipitation occurs, at which point it is discarded. Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water. Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water. Working BSA concentration range: 0.5 – 1.25 mg/ml Method 1. Prepare the folloving test tubes: Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.25 mg/ml BSA 0.1 ml 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 ml 1.0 mg/ml BSA 0.1 ml 1.25 mg/ml BSA 0. 1ml Unknown 0.1ml protein Working 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml Bradford 2. After each addition of the working Bradford reagent, thoroughly mix the…Complete the table below. Assume that the only reaction is dissolution. Ignore the effects of acid/base hydrolysis. Show complete solutions. Show both the rounded and non-rounded answers. For the rounded answer, express in exactly 5 significant figures. Salt Ksp Common ion [cation] [anion] AgX 2.24 х 103 М х 5.27 х 109м Hg2X2 1.33 x 102 M Hg2* 1.65 x 107 MExplain the mechanism of denaturation for the following agent: 1. Hydrochloric acid 2. Lead (II) acetate solution 3. Picric acid 4. Nitric acid 5. Ethanol
- What is the iodine indicator test used for? Describe in detail how iodine works in a reaction and what results are producedResults and Discussion: Folin's McCarthy Sullivan Test: Samples: proline, methionine, alanine Reagents: sodium hydroxide (5N), Glycine (1%) and 10% sodium nitroprusside solution, 6N HCl -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (5N), followed by addition of few drops of glycine (1%) and 10% sodium nitroprusside solution (HANDLE WITH CARE)and vortex. Place the test tube in a hot water bath, maintained at 40 C, for 15 minutes. Cool the test tube in ice cold water for 5 minutes and add 0.5 ml of 6N HCl. Vortex the contents and allow to stant fpr 15 minutes at room temperature.The above molecule was subjected to acid hydrolysis then was analyzed thru qualitative tests. Indicate whether the given molecule will give a positive (+) or negative (-) result in each given test. Identify whether (+/-) Test for phosphate: Dische Test: Bials Test: Murexide test: Wheeler-Johnson Test: 6. What is the transitory temperature?: Higher or lower?
- Which ane of the following reagents cannot be used for determination of lipid concentrations? Folch's reagent Bloors reagent Aniline reagent 03008 Phosphovanillin reagentA positive result for the Ninhydrin test yields a deep blue or violet-blue color of the solution, more commonly called as "Ruhemann's purple". What are the reactants involved in the formation of this violet-blue condensation product? O Oxidized ninhydrin + ammonia + another ninhydrin molecule O Reduced ninhydrin + ammonia + another ninhydrin molecule O Reduced ninhydrin + ammonium + another ninhydrin molecule Reduced ninhydrin + ammonium + an oxidized ninhydrin moleculeComplete the following precipitation reactions using balanced chemical equations: