Bacterial Morphology
The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology.
Bacterial cell structure
Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.
What is the purpose of the alcohol (OH) in the
Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under microscope. So researcher does staining before identifying them under microscope.
Staining is a technique, which is used to enhance the contrast in samples. The steps of the staining are very important and need to do very carefully. Such as smear, applying stain, air dry, heat-fixed, addition of mordant, alcohol wash, water wash, blotting, etc.
Gram-staining is a very common type of staining, which is used to differentiate bacteria based on their different cell wall constituents. It can differentiate gram-positive and gram-negative bacteria.
- Gram-positive bacteria- have thick layer of peptidoglycan layer in their cell wall, and for that reason they stain violet (crystal violet).
- Gram-negative bacteria- have thin peptidoglycan layer in their cell wall, for that they stain red after gram staining.
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