Time (min) 0 10 20 Volume change (mL) Oxygen Consumption Tube 1 Germinating Soybeans (mL/min) 8.5 6.2 3.9 I Tube 2 Nongerminating Soybeans Fluid Level (mL) 8.2 8 7.8 Volume change (mL) = Initial Volume - Final Volume Germinating Beans Tube 3 Glass Beads 5.5 5.5 5.5 Non-Germinating Beans
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- SUBSTRATE CONCENTRATION [S] µM INITIAL VELOCITY V0 s-1 10 0.13 25 0.27 50 0.45 100 0.65 150 0.77 200 0.85 300 0.94 500 1.03 (i) a) Construct an empty table with the following column headings: Substrate concentration [S] and initial velocity (Vi) where [S] has the unit µM, and Vi has the unit mM/s. (ii) The table provided is the enzyme kinetic data for your mutated enzyme, whereby Vi was expressed using the unit ∆A(405 nm)/s. Using the standard curve, express Vi with the unit mM/s rather than ∆A(405 nm)/s. Place your answer in the table above alongside the appropriate [S]. Hint: To answer this question you need to use this standard curve equation=0.0419x (The slope of the line is= 0.0419) (iii) The unmutated form of your protein has a Km of 25 µM and a Vmax of 43 mM/s. The enzyme kinetic data for your enzyme with the amino acid substitution should now be displayed in the table above. Based on these data, what is Vmax? Km? and…6.1 EXPECTED RESULT FOR CELL COUNT USING HAEMOCYTOMETER Table 2 Number of cell in upper grid 15 13 Grid location number Number of cell in lower grid 20 17 1 3 4 10 14 18 13 7 20 15 17 14 13 10 10 12 13 18 Total cells in upper and lower grid 120 120 TOTAL CELLS 240 Total grids 16 Please calculate a numbers of cell/ml from the information table 2.21 500 Density O x Safari - Jan 20, 20. X Untitle Density ConversionVo. x UnitConversionSE.. x MeasuringVolume. A. What is the starting volume of water in the graduated cylinder? B. Place the apple into the cylinder. What is the volume in the cylinder how? The water rises in the cylinder because it is displaced, or pushed upward, by the apple. The amount of displaced water is equal to the volume of the apple. C. What is the volume of the apple? (Use the Object volume to check.) Note: Milliliters (mL) are used for liquid volumes, while cubic centimeters (cm") are used for solids. One mL is the same volume as one cm". The apple's volume is measured in cm". Get the Gizmo ready: Activity A: • Replace all objects on the shelf. • Be sure the liquid in the beaker is Water. Sink or float? Question: How do mass and volume affect sinking and floating? 1. Predict: Which objects do you think will float in water? Which do you think will sink? Record your predictions below in the first column of the…
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.Give typed solution only Calculate the ML/HR (rate) Merrem 1 g (1g/50 mL of 0.9% NACL) IVBP Q 12 hours Infuse over 30 minutes. *Please show how you would work this using dimensional analysis, if possible. If not, it's fine if you show how you arrived at your answer another way*
- Only need help with 1 D and 2 DQuestion: Calculate the Rate Ratio of Giardia among hikers that filter water compare to hikers that don't filter water. 1.00 0.23 0.33 0.11 E+ = filter E-= no filter D+ = Giardia D-= no Giardia Patient Number E+1 E+ 2 E+ 3 E+4 E-5 E-6 E-7 E-8 1 X 2 3 Day X 4 X X 55 tabs po qd x4 d, taper down by one tablet every 5 d for 15 d then ss po qd. How many tablets will the patient need to take after the taper per day?