This scientist provided evidence that spontaneous generation does not occur by showing that the boiled contents of a flask in which a curved, open neck admitted air but not particles showed no signs of life. Pasteur Spallanzani Koch Redi
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- Pasteur’s experiments on spontaneous generationcontributed to the methodology of microbiology,understanding of the origin of life, and techniques for thepreservation of food. Explain briefly how Pasteur’sexperiments affected each of these topics.Use the following diagram to explain how Pasteur’s swan-necked flasks prevent contamination of sterile broth the flasks.Describe what happens to the sterile broth (a) after it has beenallowed to cool as in (b). What happens to the broth after theflask has been tipped enough to let the broth come in contactwith the dust and microorganisms and is tipped back as in (c)?Pasteur’s experiment on spontaneous generation contributed to the methodology ofmicrobiology, understanding of the origin of life, and technique for the preservation offood. Explain briefly how Pasteur’s experiments affected each of these topics.
- Explain the principle behind the Pasteur flask instudies on spontaneous generationExplain the Pasteur effect on the aerobic culture of yeast on glucose, where the rate of glucose consumption declined dramatically.Based off Pasteur’s sterility experiment, which of the following choices is least likely to result in microbial growth? Nutrient broth placed in flask, heated, swan neck removed Nutrient broth placed in flask, not heated, swan neck not removed Nutrient broth placed in flask, not heated, swan neck removed Nutrient broth placed in flask, heated, swan neck not removed
- Describe the experiments Pasteur used to disprove theidea of spontaneous generationWhat did Pasteur's results show? ► View Available Hint(s) When growth medium is not contaminated by preexisting cells, cells still grow there. When growth medium is sterilized by boiling, it remains sterile even if new cells are introduced. When growth medium is not contaminated by preexisting cells, the growth medium remains sterile. O With enough time, any sterile environment will become contaminated with cells.How would the presence of endospores in Louis Pasteur's nutrient solutions have affected his conclusions about spontaneous generation?
- It is a couple of years in the future and you have taken a job as a WSU lab prep assistant. You are cleaning up after a group of MICR 2054 students. They have been using Bacillus subtilis, a species that produces endospores. Which of the following techniques would you use to clean their glassware? Group of answer choices Boiling Pasteurization Autoclaving Any of the listed methods. Pasteurization, autoclaving, and boiling are all equally effective against endosporesHelp indetifying the unknown bacteria ? Gram Stain: Pink Cell Morpholgy: Bacillus Oxygen Requirement: OA Acid Fast Stain: green Endospore Stain: pink rods Blood agar-hemolysis: lysis (yellow clearing) Phenol red-glucose fermentation:red,no gas Phenol red -lactose fermentation:red,no gas Phenol red - mannitol fermentation:red,no gas Phenol red-sucrose fermentation: red,no gas Methyl red:no change Voges-Proskaur: no change Oxidase: purple Catalase: bubbles Nitrate Reduction: red after zinxHelp indetifying the unknown bacteria ? Gram Stain: Purple Cell Morpholgy: Rods Oxygen Requirement: OA Acid Fast Stain: pink Endospore Stain: pink rods Blood agar-hemolysis: no lysis Phenol red-glucose fermentation:red,no gas Phenol red -lactose fermentation:red,no gas Phenol red - mannitol fermentation:red,no gas Phenol red-sucrose fermentation: red,no gas Methyl red:no change Voges-Proskaur: no change Oxidase: no change Catalase: bubbles Nitrate Reduction: red after a & b