The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.2P
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Transcribed Image Text:The standard curve to determine the
amount of betacyanin is shown
below.
You extracted a red pigment from a
beet disc (the mass of a disc is 2 g)
using 10 ml of 20% ethanol solution.
You measured absorbance of the
solution above the beet disc every
minute for a total time of 20 minutes.
The increase in absorbance was
linear during a period of time from 1
min to 10 min. The absorbance at 10
min was 0.8. Calculate the amount of
betacyanin extracted from 1g of a
beet tissue per minute.
Explain your calculations. You can
use Excel or a calculator.
Transcribed Image Text:Explain your calculations. You can
use Excel or a calculator.
Absorbance at 550nm
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
0
.........
y = 76.222x + 0.0501
0.005 0.01 0.015 0.02
Concentration of Pure Betacyanin (mg/ml)
0.025
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