The following stock solutions are available to make a protein extraction buffer: 100% Nonidet P-40, 1 M Tris-Cl, and 0.5 M EDTA. What quantity of the original stocks will be needed to make 250 ml of buffer with the following final concentrations: 0.5% Nonidet, 150 mM Tris-Cl, and 10 mM EDTA?
Q: In a folded protein, Glu116 is close in three-dimensional space to Lys224. The pKa of the carboxylic…
A: pKa is the pH at which a weak acid is 50% dissociated. Alternatively, pKa = -log Ka. In acid-base…
Q: The reaction S-> P is catalyzed by an enzyme that follows Michaelis-Menton kinetics. From the…
A: The rate of enzyme catalyzed reaction that is the enzyme kinetics can be expressed by the…
Q: Which of the following is/are true about Okazaki fragments? 1. Okazaki fragments are found in the…
A: DNA replication is semi-conservative. The double helix of DNA unwinds during DNA replication and…
Q: Match each type of molecule with the corresponding description. Answer choices may be used only…
A: The biological macromolecules can be classified as nucleic acids, proteins, carbohydrates and…
Q: Consider the following experimental data from another experiment: [S] 1.5 2.00 2.50 5.00 10.00 V (No…
A: Parameters such as Km and Vmax are used for comparing enzyme activities. If we know the initial rate…
Q: In humans, the biosynthesis of L-glutamine is catalyzed by the L-glutamine synthetase. The global…
A: A negative ∆G value for a reaction implies that the reactants, or initial state, have more free…
Q: 1.b)Which one of the following would produce the most reliable evidence? in vitro study case report…
A: There are several different types of study designs that are used to gather evidence, each with its…
Q: In protein structure, which is not established via secondary interactions? o tertiary o secondary o…
A: Proteins are polymers of amino acids linked by peptide/amide bond with release of one water molecule…
Q: Passage 1 (Questions 1-4) Human immunodeficiency virus 1 (HIV-1) protease inhibitors have been…
A: Since you have posted multiple questions, we will provide the solutiononly to the first question as…
Q: What is the effect on the biosynthetic pathways if the oxidative branch of a person’s pentose…
A: The pentose phosphate pathway (PPP) is an alternate pathway to metabolize Glucose 6-phosphate…
Q: A genetically modified bacterium was cultivated to produce 1,3-propanediol (1,3-PDO), and the…
A: Oxygen Demand is a measure of the amount of oxidizable substances in a water sample that can lower…
Q: Which energy system did you use for a vertical one-clap pushup or pushup? (Choose one) A) ATP-PC…
A: Answer ATP-PC system Explanation The ATP-PC system, also known as the phosphagen system, is the…
Q: Qualitative Tests for Carbohydrates: Sugar Solution Glucose Sucrose Lactose Hydrolysis: Molisch's…
A: Qualitative tests for carbohydrates are done to confirm the presence of carbohydrates in a sample,…
Q: Why was it necessary to use phosphoaminophosphonic acid-adenylate ester (ANP) in the ATP synthase…
A: As per the chemiosmotic Model, ATP synthase is the enzyme that phosphorylates ADP with Pi as…
Q: 2. A mutation that changes an alanine residue in the interior of a protein to valine (A157 → V157)…
A: Different protein gain its three dimensional structure after folding, which occurs according to the…
Q: 8. The amino acid sequence for the beginning of the globular protein myoglobin is:…
A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…
Q: Sugar Molisch's Solution Test Glucose Sucrose Lactose Hydrolysis: Samples Sucrose hydrolysate Starch…
A: Glucose , sucrose, lactose, starch all are carbohydrates. Glucose is the simplest carbohydrate of…
Q: Design an assay with purified phycocyanin to test protein stability using 500ml of 95% ethanol. Then…
A: Phycocyanin is a pigment that is found in cyanobacteria and algae. It’s a member of the…
Q: Which of the following scenarios would ONLY occur if your skipped the digest purification step?…
A: Restriction enzymes are enzymes that cleave DNA at specific sites, and are commonly used in…
Q: Identify different types of organic reaction mechanism (SN1, SN2, etc) and reaction types (addition,…
A: SN1 reactions are substitution nucleophilic reactions that occur in a single step. They involve the…
Q: TRUE OR FALSE 1. FMN or FAD is the semiquinone form of FADH2. 2. Coenzymes can be classified based…
A: Enzymes are biological catalysts that catalyse biochemical reactions. Most enzymes are made up of…
Q: This reaction can occur without a catalyst, in the presence of a chemical catalyst (platinum) or of…
A: 1. Catalyst, both biological and chemical ones. increase the rate of a reaction without influencing…
Q: (d) NH + (a) CH₂ CH₂ (b) O-H-- CH H-C CH₂ CH3 CH₂COOH H H CH₁ CH, (c) 5 5 (b) CH₂ CH₂ CH₂ CH CH (d)…
A: Proteins are the macro molecules that show diversity in their structure by possessing four levels of…
Q: Describe how each of the following relates to protein configuration: primary structure - secondary…
A: As per the central dogma of molecular biology, DNA contains the code that is necessary for the…
Q: Why does casein precipitates upon the addition of acetic acid? Why is milk used as an antidote in…
A: Proteins are primarily composed of one or more polypeptide chains. Every polypeptide chain is a…
Q: (c) Outline how you would investigate whether BCMAP would be an effective inhibitor for the protein…
A: Inhibitors are the molecules that slow down or completely block the protein activity of molecules,…
Q: What product is formed in the committed step of the biosynthesis of pyrimidines? O a. pyrophosphate…
A: Pyrimidine biosynthesis happens in the cytoplasm with six steps process and it is synthesized from a…
Q: 4. At pH 9.5, what is the net charge of the peptide LTDQRHGE?
A: Peptide is composed of polymer of amino acids (length of 13 to 17 amino acids) which is linked by…
Q: Explain the role of carnitine in the energy supply of the myocardium
A: Carnitine plays an important in heart's metabolism. It's responsible for transporting fatty acids,…
Q: An enzyme facilitate catalysis by formation of ester bond with an alcoholic substrate. Which amino…
A: Ester bond: The bond that is formed by the condensation of alcohol group with a carboxylic acid…
Q: The measurement of alanine aminotransferase activity (reaction rate) usually includes an excess of…
A: If a particular reaction brings about a measurable change (like the generation or consumption of a…
Q: The graphs 3 and 4 representing 1/Vo = f(1/[S]。) have been done in the presence of a competitive…
A: Enzymes carry out most of the processes required for life. They are protein in nature and carry out…
Q: 1. Biochemical mechanisms of fatty acids and glycerol mobilization. 2. Metabolism of glycerol,…
A: Triglycerides are fatty acid esters of glycerol, where three fatty acid molecules are linked to one…
Q: 1. 2. 3. Why does casein precipitates upon the addition of acetic acid? Why is milk used as an…
A: Casein is a phosphoprotein found in milk and so also called as milk protein. It has an isoelectric…
Q: As we grow up or old, metabolism gets faster or slower?
A: Metabolism is the process that involves catabolism and anabolism . These are the reactions during…
Q: 3. With the help of the internet and your textbook, hand-draw the chemical structures of the…
A: Drugs are chemical substances that are used to treat infection, disease or a wound. Drugs are…
Q: What is the concentration of the cough suppressant in parts per million?
A: Some solutes might be present in a solution in very little amount. So, the concentration of these…
Q: Which of the following are the precursors in synthesizing myristic acid? a. 7 malonyl-CoA b. 3…
A: Myristic acid is a common fatty acid. It has 14 carbons. Its molecular formula is CH3(CH2)12COOH. It…
Q: Deduce the amino acid sequence of a polypeptide from the following: 1. Acid hydrolysis gives Ala2,…
A: Deducing the amino acid sequence of a polypeptide from the analysis of peptide fragments generated…
Q: he human body requires various nutrients which are essential for health and can be obtained from a…
A: Proteins are comprised of amino acids polymer linked by peptide/amide bonds. Fats are insoluble…
Q: With fatty infiltration of the liver, the synthesis of phospholipids is disturbed. What is the…
A: Fatty infiltration of the liver, also known as steatosis, occurs when there is an excessive…
Q: In isolating and characterizing carbohydrates, choose between starch and cellulose from a plant…
A: Carbohydrates are composed of carbon, hydrogen and oxygen (CH2O) which performs several functions in…
Q: b) How does oxygen availability determine the fate of the glycolysis pathway?
A: Glycolysis is a central metabolic pathway that is used by all cells for the oxidation of glucose for…
Q: How much 6x loading dye should be added to 49 uL of DNA? Report you answer in units of uL, with one…
A: The equation of dilution: M1xV1 = M2xV2 Where, M1 is the molar concentration of the stock solution.…
Q: Explain the localization and mechanism of acetone formation, under what physiological conditions…
A: Acetone is one of the type of ketone bodies formed in the human body. Ketone bodies are formed when…
Q: Which of the following correctly matches the building block to the macromolecule it forms? Amino…
A: Introduction There are four different types of macromolecules present in our body. These are…
Q: Sugar Molisch's Solution Test Glucose Sucrose Lactose Hydrolysis: Samples Sucrose hydrolysate Starch…
A: Qualitative tests are used to determine for the presence or absence of any substance in the given…
Q: You want to digest 1 µg of plasmid DNA in a final volume of 50 µL. Your solution containing plasmid…
A: A plasmid is an extrachromosomal DNA molecule within a cell that is distinct from chromosomal DNA…
Q: 1.a)Which one of the following diseases is caused by an iodine deficiency? pellagra goiter night…
A: Iodine deficiency is a condition in which the body does not have enough iodine to produce thyroid…
Q: 3. Please draw the core structure of Lignan, and numbering the C-atoms.
A: In 1948, Haworth first introduced the term "Lignan", which are also subgroup of non-flavonoid…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- The protein concentration of a known standard is 100mg/mL If you prepared a serial dilution, mixing 10μL of protein with 40μL of water what would be concentrations of the first 3 dilutions?To prepare a gel sample, you want to load 50 ng total of protein/well. You have added 200 μL of protein in 800 µL reagent and measured your sample by Bradford A595 to be 0.7 mg/mL - your dilution is unaccounted for at this point. Assuming a total final volume of 20 μL, what volume of protein sample, buffer and 4X SDS PAGE Loading Dye needs to be mixed to create a final sample of 50 ng protein in 20 μL?A stock solution contains 0.2 mg/mL protein. From this stock solution, 179 uL is diluted with 1.1 mL buffer. What is the concentration of protein in this dilution?
- A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show workingAn ion-exchange chromatographic separation is performed using a diethyl-aminoethyl- (DEAE)-sepharose column to separate proteins in a mixture. The mixture contains Protein A (pl=6.0), B (pl35.0), C (pl=7.5), D (pl =1), and E (pl=4.0). The protein mixture is prepared in a buffer solution pH =5. When the protein mixture is loaded onto the column, and the column is washed with a buffer solution pH 5, which protein(s) will be captured by DEAE-sepharose in the column? O Protein B because it is predominantly in net negative charge form. O Proteins D and E because they have predominately net negative charge in pH 5 solution O Proteins A, C, D and E because they have charges O Proteins A and C because both both predominantly have net positive charges O Proteins Band E because both predominantly have net positive chargesIn an experiment, separation of albumin from chicken liver was attempted at 31%, 58%, and 65% saturation with ammonium sulfate, (NH4)2SO4. After homogenization and centrifugation, 23 ml of the supernatant was obtained. What is the volume of saturated buffered (NH4)2SO4 (pH 7.0) that must be added to make the solution 31% saturated? 10.3 ml 8.33 ml 9.50 ml O 12.2 ml
- You need to make a protein buffer of: . • • 100 mM NaCl 25 mM Tris 8 5% w/v glycerol • 2 mM DTT Your stock solutions are: 5 M NaCl • 2 M Tris 8 • 70% w/v glycerol • DTT @ 154.25 g/mol How would you make a 1L protein buffer Solution? Show your work and describe.Laemmli buffer, also referred to as SDS-reducing buffer, is added to each of the samples. It is a blue solution and the recipe for 2X Laemmli buffer is: 0.125 M Tris HCl, pH 6.8, 20% glycerol, 4% SDS and 10% β-mercaptoethanol (βME). What is the purpose of each of these ingredients?A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?
- If I want to make a 100mL buffer that has 2% SDS, 100mM tris pH 8, 10mM EDTA, and proteinase K [1ug/ml]; How much of each would I need to add to create the buffer?Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.amino glycine pka is 2.4, 9.8 Calculate the most effective buffering range. if there is two pka, since there are 2 buffer region, is the buffering range 1.4~3.4 and 8.8~10.8? i would like to know the correct answer and detailed solution/reason.