The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m 6- RNA Absorbance at 260 (µg) 0.00 10.29 20.58 30.87 41.16 mass= nm 0.227 0.452 0.786 1.078 1.296 1g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.523. ug
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m 6- RNA Absorbance at 260 (µg) 0.00 10.29 20.58 30.87 41.16 mass= nm 0.227 0.452 0.786 1.078 1.296 1g-1 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.523. ug
ChapterU5: Fire: Energy , Thermodynamics, And Oxidation-reduction
SectionU5.116: How Absorbing: Spectroscopy
Problem 6E
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