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The chromatogram in the figure is of a mixture of 2 compounds, carried out in a packed liquid chromatographic column of 25 cm length. The flow rate was 0.40 mL min-¹. A species that is not retained passes through the column in 5 minutes. a) Determine the retention factors for the two components. b) Calculate the resolution of the two peaks. c) Find the average height of the plate. d) What column length would be needed to achieve a resolution of 1.75? Detector response 0 5.0 A 22.0 20 30.0 30 Time, min 38.0 40 39.0 50 50.0 60 62.0 70

The chromatogram in the figure is of a mixture of 2 compounds, carried out in a packed liquid chromatographic column of 25 cm length. The flow rate was 0.40 mL min-¹. A species that is not retained passes through the column in 5 minutes. a) Determine the retention factors for the two components. b) Calculate the resolution of the two peaks. c) Find the average height of the plate. d) What column length would be needed to achieve a resolution of 1.75? Detector response 0 5.0 A 22.0 20 30.0 30 Time, min 38.0 40 39.0 50 50.0 60 62.0 70

Principles of Instrumental Analysis
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
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The chromatogram in the figure is of a mixture of 2 compounds, carried out in a packed liquid chromatographic column of 25 cm length. The flow rate was 0.40 mL min ¹. A species that is not retained passes through the column in 5 minutes. a) Determine the retention factors for the two components. b) Calculate the resolution of the two peaks. c) Find the average height of the plate. d) What column length would be needed to achieve a resolution of 1.75? Detector response 0 5.0 10 22.0 20 30.0 30 Time, min 38.0 40 39.0 50 50.0 60 62.0 70
Transcribed Image Text:The chromatogram in the figure is of a mixture of 2 compounds, carried out in a packed liquid chromatographic column of 25 cm length. The flow rate was 0.40 mL min ¹. A species that is not retained passes through the column in 5 minutes. a) Determine the retention factors for the two components. b) Calculate the resolution of the two peaks. c) Find the average height of the plate. d) What column length would be needed to achieve a resolution of 1.75? Detector response 0 5.0 10 22.0 20 30.0 30 Time, min 38.0 40 39.0 50 50.0 60 62.0 70
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