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- Briefly explain the shake-tube inoculationConstruct a table showing the composition, advantage and disadvantage of these Platelet Diluting Fluids (1) Tripotassium EDTA and 1% Ammonium Oxalate and (2) Rees and Ecker Diluting Fluid.Explain why the following step are necessary during subculturing: Flaming the neck of tubes/bottles after capping and before re-capping?
- A) Draw and label the set-up of Manual paraffin wax infiltration. B) Answer the following questions:1. Differentiate the 3 types of tissue Impregnation in terms of: 2. Impregnation Techniques Advantages Disadvantages A. Paraffin Infiltration B.Celloidin Infiltration C.Gelatin Impregnation C) What is the required volume of embedding medium for routine tissue processing? D) What is Plastic embedding? What is Double embedding?Answer the following questions: 1. Define a bacterial colony. 2. What is the difference between macroscopic and microscopic appearance of bacteria? 3. State the three standard terms used to describe single colonies on agar plates. 4. State and define the three types of growth that may be seen in a broth culture. 5. State three basic shapes of bacteria. 6. State and describe the different arrangements of cocci. 7. What is the difference between true motility and Brownian motion?Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?
- what is MacFarland Nephelometer? How is it prepared?Explain why it is important to follow proper precautions and use aseptic technique when working with clinical samples. Why do you think that samples from urine should be tested within 2 hours of collection, or if not possible, that the sample should be refrigerated?Amswer the following questions: 1. What other physiological responses may have caused the diphasic growth in E. coli? 2.Describe the methods used by microbiologists in producing synchronous cultures from unsynchronized bacterial culture.
- Explain why the following steps are essential during subculturing:a. Flaming the inoculating instrument prior to and after each inoculation.b. Holding the test tube caps in the hand as illustrated in Figure 1.1 on page 24.c. Cooling the inoculating instrument prior to obtaining the inoculum.d. Flaming the neck of the tubes immediately after uncapping and beforerecappinghks Describe the protocol to generate competent cells through electroporation method.In what ways is the membrane filter method for coliform detection superior to the most probable number method