The Cas9 enzyme requires a short sequence called a PAM (Protospacer Adjacent Motif) for cleaving the target DNA.This PAM sequence is not found in the CRISPR locus of Streptococcus pyogenes but it is present in viral genomes and we need to make sure it is present adjacent to where the guide RNA binds the yeast DNA. What does this suggest about the function of the PAM?

Q: Does one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?

A: A molecular process in which some discontinuous change to nucleotide sequences in their genomic DNA…

Q: Imagine that you completed your ligation of the gRNA insert (annealed oligos) into cut pML104 vector…

A: Nuclear localization signals (NLS) is importnat because it helps to transport Cas9 into the nucleus…

Q: In a single-locus homology-directed repair (HDR), CRISPR/Cas9 machinery makes breaks in the dsDNA at…

A: 2

Q: Like many biotechnologies, CRISPR/Cas9 was originally discovered as system that naturally occurs in…

A: Biotechnology is the branch of biology which deals with the utilisation of cells and biomolecular…

Q: Briefly describe two approaches that are used to edit the germline in embryos using CRISPR-Cas9.…

A: The mechanism of CRISPR/Cas-9 genome editing can be generally divided into three steps: recognition,…

Q: It has been shown that infectious agents such as viruses often exert a dramatic effect on their host…

A: Viruses are the obligate parasites that take over the machinery of their host cells.

Q: How can the construct with Cas9 and gRNA sequence be removed from the plant in case it was inserted…

A: The generation of new plants with improvised constructs of features that are desirable is based on…

Q: In the study of the SARS-CoV virus protein codes, it was discovered that eight potential open…

A: Answer: SARS-CoV and MERS-CoV = These are the infectious respiratory diseases causing viruses.…

Q: Previous research had found odd sequences, spacers, and repeats, within some bacterial genomes.…

A: It is the spacers that enable the adaptable and gene-specific inactivating mechanism of the CRISPR…

Q: Which of the following statements about the CRISPR-Cas9 technique is correct? 1. Catalytically…

A: CRISPR-Cas9 (Clustered regularly interspaced short palindromic repeats-CRISPR associated protein) is…

Q: Sometimes, cDNAs turn out to be “chimeras”; that is, fusions of DNA copies of two different mRNAs…

A: c dna is complementary dna.

Q: The clustered, regularly interspaced short palindromic repeats (CRISPR) system is like a bacterial…

A:

Q: In type II CRISPR-Cas9 systems, what intermediate interacts with the repeat sequences that are added…

A: CRISPR Cas 9 edits genes by precisely cutting DNA and then letting natural DNA repair processes to…

Q: QuestionHuman ribosomes can translate the SARS-CoV2 genome because:   A.  it is reverse…

A:

Q: Which of the following is a correct statement about CRISPR-Cas-9 gene editing? Group of answer…

A: CRISPR-Cas9 is a widely used genome editing technology as it enables scientists to alter genes in…

Q: During a CRISPR-Cas9 exp., yeast are transformed with PML104-9RNA1 plasmid and HDR1 template to edit…

A: CRISPR/Cas system is a revolutionary technology that provides insights into the development of an…

Q: One problem that researchers sometimes encounterwhen editing genomes with CRISPR/Cas9 is that oneor…

A: The gene therapy or gene editing is an asset for humans, as many of the gene-related disorders can…

Q: You were able to obtain the RNA of the influenza virus. If you injected this into a susceptible host…

A: The influenza viruses have segmented, negative-strand RNA genomes that require replication by an…

Q: Discuss the genetic switch that controls the lytic versus lysogenic path in bacteriophage lambda.

A: Gene regulatory network that shows bistable behavior is known as genetic switches. It is studied…

Q: In prokaryotes, the sigma factor recognizes base sequences in the _____ which facilitates RNA…

A: Prokaryotes are unicellular organisms that lack a true nucleus. Their genetic material is not…

Q: Here is a partof the mRNA sequence of the spike glycoprotein of the SARS Covid-19 virus, starting…

A: A polypeptide chain is made by the process of translation wherein the mRNA sequence of base pairs is…

Q: You have cloned a novel E. coli gene and wish to express protein from it in human cells. In order to…

A: The Central Dogma theory explains that DNA forms DNA by Replication, DNA forms RNA by Transcription…

Q: How will this modified cro protein interact with the three OR sites, and how would cro expression be…

A: Bacteriophages are viruses that infect bacteria. Like other viruses bacteriophages are also made up…

Q: What would a scientist need to use CRISPR-Cas system to introduce a specific point mutation into a…

A: When the target DNA is located, Cas9, one of the CRISPR system's enzymes, latches to it and chops…

Q: What is the actual biological purpose of the CRISPR/Cas9 system?

A: All biological information of an organism is stored inside the biomolecule called DNA.  Gene are the…

Q: Suppose you cultured yeast, that you had transformed with pMZ379 plus the AVT5 sgRNA, on a plate of…

A: Cas9 is a kind of enzyme. That functions as a pair of'molecular scissors,' allowing portions of DNA…

Q: You design a guide RNA to target your gene of interest, which you will express attached to a…

A: Cas9 It produces DNA double strand breaks that are repaired by homology directed repair (HDR)…

Q: To create a cDNA library, cDNA can be inserted into vectors and cloned. In the analysis of cDNA…

A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…

Q: You would like to design a method to treat the most common mutation that causes sickle cell anemia…

A: Precise genome editing is a potential technique for discovering causative genetic variations and…

Q: A virus is a submicroscopic infectious agent that replicates only inside the living cells of an…

A: A virus is submicroscopic infectious agent that replicates inside the living cell.  Different…

Q: CRISPR in nature evolved as a "bacterial immune system" or "genetic vaccine" to target infecting…

A: CRISPR stands for "Clusters of Regularly Interspaced Short Palindromic Repeats". Cas9 is the…

Q: Identification and quantification of expressed genes initially requires a. reverse transcription of…

A: Gene is a basic unit of heredity and a sequence of nucleotides in DNA that encodes the Synthesis of…

Q: Some DNA vaccines use a brief and small electrical shock to get DNA in plasmids into cells. What…

A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…

Q: Compare the reovirus genome to those of influenzavirus and bacteriophage MS2. Why do…

A: Reoviridae family of viruses has a wide range of hosts which include invertebrates, vertebrates,…

Q: If the RNA polymerase II subunits are in the same family, why would they have such little similarity…

A: Although RNA pol II subunits are in the same family but due to different functions they show less…

Q: The flow of genetic information is from DNA to RNA toprotein. In certain viruses, the flow of…

A: The two main steps involved in protein synthesis are: transcription and translation. These two…

Q: Based on recent data arising from various animal models, which of the following CRISPR-Cas9 systems…

A: based on recent data arising from various animal models, A. CRISPR-dCas9 systems would be the most…

Q: CRISPR/Cas9 mediated genome editing has provided an unparalleled means of manipulating the genome.…

A: CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats/CRISPR-associated genes) is a…

Q: Suppose you cultured yeast, that you had transformed with pMZ379 plus the AVT5 sgRNA, on a plate of…

A: sgRNA or single guide RNA is an abbreviation that expands as “single guide RNA.”…

Q: Retrotransposons are a class of mobile elements that transpose via an RNA intermediate. Contrast the…

A: On the basis of  their manner of mobilization, DNA transposons have the ability to move by making…

Q: Does CRISPR CAS9 as a defense in bacteria only work for double stranded viral DNA? or any type of…

A: Scientists can use CRISPR to persistently edit genes in living cells and creatures and, in the…

Q: Predict the replication step for the novel Miovirus. This virus was determined to have a dsRNA…

A: Introduction A virus is a small piece of genetic material, such as DNA or RNA, encased in a protein…

Q: The CRISPR-Cas9 system may be able to be used for somatic gene therapy to treat terminal genetic…

A: Mutation: Normal DNA contains a particular sequence of DNA. If the sequence of DNA is changed due to…

Q: Many regions of non-coding eukaryotic DNA previously thought to be "junk" are now known to contain…

A: As per the honor code, we are entitled to do only one question therefore I am providing the answer…

Q: For the sake of simplicity, Fig. 10.4 omitted one stepof cDNA library construction. The figure…

A: The process that involves annealing of single-stranded deoxyribonucleic acid (DNA) or ribonucleic…

Q: Please indicate whether the following statement is true or false In eukaryotes, promoter escape…

A:

Q: You design a guide RNA to target your gene of interest, which you will express attached to a…

A: Cas9 It produces DNA double strand breaks that are repaired by homology directed repair (HDR)…

Q: Why was the type II CRISPR-Cas9 system of S. pyogenes selected by several scientists as a model to…

A: CRISPR stands for Clustered Regularly-Interspaced Short Palindromic Repeats, is a revolutionary…