Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm Concentration of BSA Stock solution is 200 µg/mL Amount of protein in tube (ug) O ug Volume of Volume of BSA Absorbance Bradford reagent 3000 µl Cuvette no. Standard 0.00 O µl 30 µl 60 µl B 3000 µl 3000 µl 3000 µl 0.1435 1 0.2968 2 0.402 90 μι 120 µl 150 µl 0.5038 3000 µl 4 0.587 3000 µl LO

Table 1: Standard Curve for Bradford Method. Absorbance measured at 595 nm Concentration of BSA Stock solution is 200 µg/mL Amount of protein in tube (ug) O ug Volume of Volume of BSA Absorbance Bradford reagent 3000 µl Cuvette no. Standard 0.00 O µl 30 µl 60 µl B 3000 µl 3000 µl 3000 µl 0.1435 1 0.2968 2 0.402 90 μι 120 µl 150 µl 0.5038 3000 µl 4 0.587 3000 µl LO

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 14.12QAP

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