Study the illustration of the lac operon below. Normally, the genes are switched off when lactose is not present. Lactose activates the genes, which code for enzymes that allow the cell to use lactose. Mutations can alter the functions of this operon. Predict how the following mutations would affect the function of the operon the absence and presence of lactose. Mutation of regulatory gene; repressor will not bind to lactose. Mutation of operator; repressor will not bind to operator. Mutation of regulatory gene; repressor will not bind to operator. Mutation of promoter; RNAP will not attach to promoter.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Study the illustration of the lac operon below. Normally, the genes are switched off when lactose is not present. Lactose activates the genes, which code for enzymes that allow the cell to use lactose. Mutations can alter the functions of this operon. Predict how the following mutations would affect the function of the operon the absence and presence of lactose.
- Mutation of regulatory gene; repressor will not bind to lactose.
- Mutation of operator; repressor will not bind to operator.
- Mutation of regulatory gene; repressor will not bind to operator.
- Mutation of promoter; RNAP will not attach to promoter.
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