STR #1: D5S818 allele 1: 5'-CAATCATAGCCACAA | GATAGATAGATAGATAGATAGATAGATACCAAAGAG-3' allele 2: 5'-CAATCATAGCCACAAGATAGATAGATAGATAGATAGATAGATAGATACCAAAGAG-3' CSF1PO STR #2: allele 1: 5'-GGCCATCTTCAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATGCTAGTCC-3' allele 2: 5'-GGCCATCTTCAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATGCTAGTCC-3' D7S820 STR #3: allele 1: 5'-CCTCATTGACGATAGATAGATAGATAGATAGATACATAGTCAG-3" allele 2: 5'-CCTCATTGACGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATACATAGTCAG-3' STR #4: D8S1179 allele 1: 5'-GTTCATTTTCATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTACGAATGTACA-3' allele 2: 5'-GTTCATTTTCATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTACGAATGTACA-3' Locus STR #1: STR D5S818 on Chromosome 5 STR #2: STR CSF1PO on Chromosome 5 STR #3: STR D7S820 on Chromosome 7 STR #4: STR D8S1179. on Chromosome 8 Repeat unit AGAT TAGA GATA TCTA # of repeats Allele 1 01 I # of repeats Allele 2 Homozygous or Heterozygous

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter15: Genomes And Genomics
Section: Chapter Questions
Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...
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Today's technology has made it easier to quickly and accurately generate DNA profiles. In this part of the activity, you will
model the process yourself to solve a crime. Good luck, detective!
Crime Report: A thief has stolen a priceless collection of jewels from the Museum of Precious Jewels. Forensic
technicians obtained skin cells from a forehead print left on the glass enclosure of the jewel exhibit. DNA has been
isolated and PCR amplified for some of the standard STR loci. A partial genetic profile generated from the collected DNA
is shown in Figure 5.
10
50
DNA Profile from Forehead Print
Number of base pairs
00
50
40
D58818
075830
I
16
MU
DES1179
Shandand
(10)
70
CSF1PO
DITS820
80
100
Figure 5. The DNA profile of the forehead print from the scene of the crime. Each colored line shows the alleles for one of four of the
core CODIS STR loci (D5S818, CSF1PO, D7S820, D8S1179).
and data for the four STR loci that were included in the
A suspect was identified in the case. Her DNA was collecte
analysis of the forehead print were obtained. The suspect's profile data is shown on the next page.
Your task: Compare the suspect's data below to the electropherogram in Figure 5. Analyze each STR locus by locating the
repeats for each locus and counting the number of repeats and indicate whether the suspect is homozygous or
heterozygous at each locus. Record your answers on the next page.
Transcribed Image Text:1 2 Today's technology has made it easier to quickly and accurately generate DNA profiles. In this part of the activity, you will model the process yourself to solve a crime. Good luck, detective! Crime Report: A thief has stolen a priceless collection of jewels from the Museum of Precious Jewels. Forensic technicians obtained skin cells from a forehead print left on the glass enclosure of the jewel exhibit. DNA has been isolated and PCR amplified for some of the standard STR loci. A partial genetic profile generated from the collected DNA is shown in Figure 5. 10 50 DNA Profile from Forehead Print Number of base pairs 00 50 40 D58818 075830 I 16 MU DES1179 Shandand (10) 70 CSF1PO DITS820 80 100 Figure 5. The DNA profile of the forehead print from the scene of the crime. Each colored line shows the alleles for one of four of the core CODIS STR loci (D5S818, CSF1PO, D7S820, D8S1179). and data for the four STR loci that were included in the A suspect was identified in the case. Her DNA was collecte analysis of the forehead print were obtained. The suspect's profile data is shown on the next page. Your task: Compare the suspect's data below to the electropherogram in Figure 5. Analyze each STR locus by locating the repeats for each locus and counting the number of repeats and indicate whether the suspect is homozygous or heterozygous at each locus. Record your answers on the next page.
STR #1: D5S818
allele 1: 5'-CAATCATAGCCACAA | GATAGATAGATAGATAGATAGATAGATACCAAAGAG-3¹
allele 2: 5'-CAATCATAGCCACAAGATAGATAGATAGATAGATAGATAGATAGATACCAAAGAG-3'
STR #2: CSF1PO
allele 1: 5'-GGCCATCTTCAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATGCTAGTCC-3'
5'-GGCCATCTTCAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATGCTAGTCC-3'
allele 2:
D75820
STR #3:
allele 1:
allele 2: 5'-CCTCATTGACGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATACATAGTCAG-3'
5'-CCTCATTGACGATAGATAGATAGATAGATAGATACATAGTCAG-3"
STR #4:
allele 1:
allele 2:
D8S1179
5'-GTTCATTTTCATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTACGAATGTACA-3¹
5'-GTTCATTTTCATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTACGAATGTACA-3'
Locus
STR #1: STR D5S818
on Chromosome 5
STR #2: STR CSF1PO
on Chromosome 5
STR #3: STR D7S820
on Chromosome 7
STR #4: STR D8S1179.
on Chromosome 8
SUSPECT'S DNA PROFILE DATA
Repeat
unit
AGAT
TAGA
GATA
TCTA
# of repeats
Allele 1
I
# of repeats
Allele 2
Homozygous or Heterozygous
Transcribed Image Text:STR #1: D5S818 allele 1: 5'-CAATCATAGCCACAA | GATAGATAGATAGATAGATAGATAGATACCAAAGAG-3¹ allele 2: 5'-CAATCATAGCCACAAGATAGATAGATAGATAGATAGATAGATAGATACCAAAGAG-3' STR #2: CSF1PO allele 1: 5'-GGCCATCTTCAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATGCTAGTCC-3' 5'-GGCCATCTTCAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATGCTAGTCC-3' allele 2: D75820 STR #3: allele 1: allele 2: 5'-CCTCATTGACGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATACATAGTCAG-3' 5'-CCTCATTGACGATAGATAGATAGATAGATAGATACATAGTCAG-3" STR #4: allele 1: allele 2: D8S1179 5'-GTTCATTTTCATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTACGAATGTACA-3¹ 5'-GTTCATTTTCATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTACGAATGTACA-3' Locus STR #1: STR D5S818 on Chromosome 5 STR #2: STR CSF1PO on Chromosome 5 STR #3: STR D7S820 on Chromosome 7 STR #4: STR D8S1179. on Chromosome 8 SUSPECT'S DNA PROFILE DATA Repeat unit AGAT TAGA GATA TCTA # of repeats Allele 1 I # of repeats Allele 2 Homozygous or Heterozygous
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