Starch is a polysaccharide 1.Is starch positive in Bial‘s test?(what color ?) 2.Is starch positive in (Seliwanoff‘s)ketohexoses test?(what color would it be if negative)

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Chapter15: Antibiotic Agents
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Starch is a polysaccharide 1.Is starch positive in Bial‘s test?(what color ?) 2.Is starch positive in (Seliwanoff‘s)ketohexoses test?(what color would it be if negative)
۸۰۰ сотсиол
tu2. Bial's Test (Distinguish between Pentoses and Hexoses) ibensbb selgrad
To test for pentoses, add 0.5 mL of 1% carbohydrate solution (in water) to 1 mL of
Bial's reagent, (Use a glass Pasteur pipette to add the Bial's reagent: do not use a
mechanical pipettor with concentrated acids) and heat the solution in a boiling
water bath for 2 minutes. A blue-green color indicates pentoses or nucleotides
containing pentoses; a yellow-green color indicates hexoses, and dísaccharides
are yellow.
ceas Yellow Blue
zdrops.
groeps
3. Resorcinol (Seliwanoff's) Test (Ketohexoses) A-hoqs
To test for ketohexoses, add 0.1 mL of a 1% carbohydrate solution in water to 119
mL of the reagent, and heat the solution in a boiling water bath for 5 minutes. A
deep red colored precipitate within 5 minute indicates ketohexoses. Sucrose gives
a positive ketohexose test because of partial hydrolysis to glucose and fructose.
Other sugars give a red color upon prolonged heating.onomonengont
Structure of the
Shu the
neay. Rection..
"unknowns. Why Blue
one sugalwoduct.
н-с-но
1
INV
Transcribed Image Text:۸۰۰ сотсиол tu2. Bial's Test (Distinguish between Pentoses and Hexoses) ibensbb selgrad To test for pentoses, add 0.5 mL of 1% carbohydrate solution (in water) to 1 mL of Bial's reagent, (Use a glass Pasteur pipette to add the Bial's reagent: do not use a mechanical pipettor with concentrated acids) and heat the solution in a boiling water bath for 2 minutes. A blue-green color indicates pentoses or nucleotides containing pentoses; a yellow-green color indicates hexoses, and dísaccharides are yellow. ceas Yellow Blue zdrops. groeps 3. Resorcinol (Seliwanoff's) Test (Ketohexoses) A-hoqs To test for ketohexoses, add 0.1 mL of a 1% carbohydrate solution in water to 119 mL of the reagent, and heat the solution in a boiling water bath for 5 minutes. A deep red colored precipitate within 5 minute indicates ketohexoses. Sucrose gives a positive ketohexose test because of partial hydrolysis to glucose and fructose. Other sugars give a red color upon prolonged heating.onomonengont Structure of the Shu the neay. Rection.. "unknowns. Why Blue one sugalwoduct. н-с-но 1 INV
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