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Separation of Amino Acids by Thin Layer Chromatography Lab Questions. Thank you for your help on these questions!
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- 6) You are given a mixture of organic compounds suspected of containing various amines (e. g. 4-chloroaniline). What is the best way to quickly separate these amines from the other organic compounds? a) Perform a fractional distillation on the mixture. b) Benchtop silica gel column chromatography. c) Dissolve the mixture in an organic solvent and treat the solution with aqueous HCI. d) Dissolve the mixture in an organic solvent and treat the solution with aqueous NaOH.In a chromatography experiment measuring the polarity of amino acid funtional groups, one amino acid had an Rf value of 0.32 and another had an Rf of 0.68 ‐‐‐ which one traveled furthest on the chromatography paper?1. A student separated the three compounds below using the acid base extraction procedure. He then followed is experiment with TLC using acetone hexanes mixture as mobile phase. What would you expect the student to see on TLC? Would he run into difficulty with the TLC? Explain octahydro-1H-indole Lauric Acid OH NH₂ cyclohexanecarboxamide
- Does the presence of a benzene ring or carboxylic acid functional group in a substance affect the retention factor (Rf) when doing TLC on silica gel ?A thin-layer chromatography (TLC) experiment was run using three compounds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with these compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?What are the chief differences between the Ninhydrin and Bradford assays for amino acid quantification? Why would one technique be preferred over the other?
- 1. Two of the substances used in this experiment, acetyl and 1,1l'-diacetylferrocene, contain the acetyl group. Draw an acetyl group and the compound acetyl chloride. What is the difference between an acetyl and acyl group? 2. During the chromatography the solvent may be changed from hexanes/ ethyl acetate (8:2) to hexanes/ ethyl acetate (4:6) depending on which ferrocenes are present in the mixture. (a) What is the reason for doing this? (b) Which of these solvent mixtures is more polar? (c) How would you prepare 200 mL of hexanes/ ethyl acetate (8:2)?In what order would the following compounds be eluted on a silica gel TLC plate if they are spotted on the same plate and developed in a solvent mixture of 10:1 hexanes:ethyl acetate A. 1 has the highest Rf value, then 2 then 4 then 3 b. 4 has the highest Rf value, then 1 then 3 then 2 c. 3 has the highest Rf value, then 2 then 1 then 4 d. 2 has the highest Rf value, then 3 then 1 then 4The surface of the silica gel has a structure that looks like this diagram below. Suppose you used a plate coated with silica gel, with propanone, CH3COCH3, as the solvent for thin-layer chromatography. Sno Suppose also that the mixture you were trying to identify contained • benzaldehyde •benzanilide • phenol 1) rank the Rf Value from large to small 2) name the IMF between each compound and the silica gel OH OH main body of silica structure
- Identify the statement that correctly interprets this TLC analysis of a recrystallized sample of acetaminophen produced following the procedure outlined in Experiment 5: The TLC of an individuals purified acetaminophen showed two spots. One spot had an Rf identical to that of pure acetaminophen. This spot was also larger and darker than the second spot. The Rf of the second spot was very close to the Rf of pure 4-aminophenol. a) The two spots evident in my TLC analysis indicate my sample is a mixture. Since each spot has an Rf value that is close to the Rf of one of the authentic materials tested, I can conclude my sample is a mixture of acetaminophen and 4-aminophenol. The large size of the spot with an Rf nearly identical to authentic acetaminophen indicates my sample is mostly 4-aminophenol. b)I can conclude from this evidence that my sample is a mixture of acetaminophen and 4-aminophenol because the Rf of each of the spots is nearly identical to one of the authentic…Considering the Rf’s of acetaminophen, aspirin, & caffeine, which compound has the most favorableinteractions with 1.)eluent 2.)silica gel?26. A mixture containing heptane, 2,2-dimethylbutane, hexanoic acid and heptanol was separated by gas/liquid chromatography. The stationary phase used was polar. In what order would the components leave the column? (A) (B) (D) Heptane, heptanol, hexanoic acid, 2,2-dimethylbutane Hexanoic acid, heptanol, heptane, 2,2-dimethylbutane 2, 2-dimethylbutane, heptane, heptanol, hexanoic acid Heptanol, 2, 2-dimethylbutane, hexanoic acid, heptane