solution is tested with three indicators and it has a pH of 9.8. Identify the colours of the following indicators: alizarin yellow R color:yellow,orange or red thymol blue: yellow,orange,blue or red bromothymol blue: yellow, green or blue
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A solution is tested with three indicators and it has a pH of 9.8. Identify the colours of the following indicators:
alizarin yellow R color:yellow,orange or red
thymol blue: yellow,orange,blue or red
bromothymol blue: yellow, green or blue
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- Bradford method Reagents Stock Bradford reagent: Dissolve 100 mg Coomassie Brilliant Blue G250 in a mixture consisting of 100ml of 85% phosphoric acid, 50ml of 95% ethanol and 50ml 1M NAOH. Store at 4°C until precipitation occurs, at which point it is discarded. Working Bradford reagent: Prepare fresh by diluting 10ml of stock Bradford reagent to 250ml with distilled water. Stock bovine serum albumin (BSA) solution (10mg/ml): Dissolve 0.2g BSA in 20ml distilled water. Working BSA concentration range: 0.5 – 1.25 mg/ml Method 1. Prepare the folloving test tubes: Table 1: Preparation of test tubes for the Bradford method Blank Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Unknown Distilled H20 1.0 ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.9ml 0.25 mg/ml BSA 0.1 ml 0.5 mg/ml BSA 0.1 ml 0.75 mg/ml BSA 0.1 ml 1.0 mg/ml BSA 0.1 ml 1.25 mg/ml BSA 0. 1ml Unknown 0.1ml protein Working 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml 4.0ml Bradford 2. After each addition of the working Bradford reagent, thoroughly mix the…Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mL
- If a sample of beeswax is found to have an acid number of 15.5 and saponification value of 71.2, the ester value of the sample is:Match the following: Schiff test Tollen’s test Fehling’s test Benedict's test Iodoform test 2,4-DNP Test Benzaldehyde Sodium nitroprusside Cuprous oxide Cupric sulfate unspecific test for carbonyls acyl substitution chromophore of N in the visible result Citrate complex oxidizing agent bounded aldehydes aromatic substitution reducing agent ketone bodies Tartrate complex Uses a basic solution/medium uses an acidic solution/medium malonate complex reduction product readily oxidizes to RC=OR form Bitartrate complex Readily oxidizes to COOH form readily oxidizes to COH formGive and explain the principles of the following tests for lipids: Spot Test Acrolein Test Test for Free glycerol Test for Surface Tension Formation of Insoluble Soap Liebermann-Burchard Test Salkowski Test
- An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?Lipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant - chloroform: methanol: water (6:3:1) 2nd eluant - methanol: chloroform (9:1) 3rd eluant – petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3,(NH4)3MoO4 3. CHCl3, acetic anhydride, conc. H2SO4 4. -naphthol, conc. H2SO4 5. Hydroxylamine HCl, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithinDiscuss why sodium hydroxide may be used in conjunction with triethanolamine to react with stearic acid in the formulation of vanishing creams.