Separation of Peaks Below are two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used?

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
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a. Separation of Peaks
Below are two chromatograms after LC separation. Which chromatogram is better to proceed for MS
analysis? What mobile phase condition/composition (LC separation) was used?
HPLC: 15% H₂O, 85% CHCN, CIS Revane Phase, 100 mm long, 5pm particles
(1)
Area (1)-14750
Arta (2)-53538
MIN
HPLC: 59% 10,49% CHCN, CIS Reverse Phase, 100 m long, 5 pm particles
(2)
Mes (1)-2256
A(2)-9536
A(3)-25638
13H
Transcribed Image Text:a. Separation of Peaks Below are two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used? HPLC: 15% H₂O, 85% CHCN, CIS Revane Phase, 100 mm long, 5pm particles (1) Area (1)-14750 Arta (2)-53538 MIN HPLC: 59% 10,49% CHCN, CIS Reverse Phase, 100 m long, 5 pm particles (2) Mes (1)-2256 A(2)-9536 A(3)-25638 13H
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