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- Researchers used gas chromatography to determine the % v/v methyl salicylate in rubbing alcohol [Van Atta, R. E.; Van Atta, R. L. J. Chem. Educ. 1980, 57, 230-231]. A set of standard additions was prepared by transferring 20.00 mL of rubbing alcohol to separate 25 - mL volumetric flasks and pipeting 0.00 mL, 0.20 mL, and 0.50 mL of methyl salicylate to the flasks. All three flasks were diluted to volume using isopropanol. Analysis of the three samples gave peak heights for methyl salicylate of 57.00 mm, 88.5 mm, and 132.5 mm, respectively. Determine the % v/v methyl salicylate in the rubbing alcohol.The partition coefficient of ammonia between water and chloroform is wanted to be determined. The necessary quantities of ingredients are taken into the separation funnel, and the extraction process is applied. At the end of the extraction process, 5 mL portions of each of the water and chloroform phases are titrated with 0.1 M HCI in the presence of methyl orange indicator. If the volumes spent at the turning points are 7.2 and 5.1 mL, respectively, calculate the KSU, KLOROFORM (Dispersion coefficient) value.A method of quantitative determination of the concentration of constituentsof a sample analyzed by gas chromatography is area normalization. Thiscomplete elution of all sample constituents is required. Thearea of each peak is measured and corrected for the detector response to the diffe- renteseluatos. This correction involves dividing the area by an empiricalcorrection factorDetermined. The concen ration of the analyte is found from the ratio between itscorrected and the total corrected area of all peaks. For a chromatogram containing threerelative areas were determined as 16.4; 45,2; and 30.2, in the order ofincreased retention time. Calculate the percentage of each compound if therelative responses from the detector are 0.65, 0.73 and 0.85, respectively.
- hree components in a mixture have varying distribution constants between the mobile phase and the stationary phass One of the components has a very low distribution constant, Would this component likely elute first or last and how would you design an experiment or modify the components of the separation to make it perform in the opposite manner?The interaction between a basic analyte and the residual silanol groups of the stationary phase is minimized when the pH of the mobile phase used is equal to the pKa of silanol. True or False?what weight of betazole phosphotungstate might be expected in the assay of 0.1550 g sample of betazole hydrochloride containing 98% C5H9N3.2HC? Gravimetric factor = 0.0909
- The following data was obtained when four bottles of wine, were analyzed for residual sugar content and the results obtained are the followingB Bottles % w/v Residual Sugar 1 0.97, 0.86, 1.02 2 1.25, 1.32, 1.13, 1.20, 1.12 3 0.9, 0.92, 0.73 0.72, 0.77, 0.61,0.58 a) For each set, determine the mean, spread, standard deviation s and coefficient of variation. b) Calculate the pooled standard deviation, spooled. 2.(2) ylubäi On the gravimetric determination of CI, if the weight of AgCl ppt. was 1.433 g.; the weight of CI- in the sample will equals to: . (AGCI ..... molecular weight = 143.3 and Cl- =35.5) 143.3/35.5 g. O 3.55 g. O 0.1433 g. O 0.355 g. OPenicillin F is recovered from a dilute aqueous fermentation broth by extraction with amyl acetate, using 6.5 volumes of solvent per 100. volumes of the aqueous phase. At pH = 3.8 the distribution coefficient KD is 74. (a) What fraction of the penicillin would be recovered in a single ideal stage? (b) What would be the recovery with three-stage extraction using fresh solvent in both stages? (c) How many ideal stages would be needed to give the same recovery as in part (b) if a counterflow cascade were used with V/L = 0.065?
- A group of two students obtain the following data for the dissolution of borax. Using the data given, calculate AH-in kJmol-1 for the for borax dissolution studied. Record answer to 2 decimal places. R= 8.314 Jmol-1K-1 Measurement 20.0 °C 50.0 °C Volume of 0.20 M HCl used (ml) 3.97 11.55 [Borate] (M) 0.0397 0.1155 Ksp 0.000250 0.006163 1/T (K-¹) 0.003413 0.003096 Type your numeric answer and submit5. Calculate the Ionic strength of solution prepared by mixing of 0.2 M NaNO3and 0.02M of K2Cr2O7:a) s = [Ksp / 27]1/5 b) s = Ksp1//5c) s = Ksp ½ d) s = [Ksp/108]1/5e) s = 108 Ksp(a) In High Performance Liquid Chromatography (HPLC), the separation method is by partition of the solute (analyte) between the mobile phase and the stationary phase. Explain how this leads to separation of analyte mixtures; (b) Describe the structure and materials use in a typical column used for HPLC. Thereby explain why high pressures have to be used in HPLC; (c) Discuss how the need for high pressures also imposes requirements on the injector;
