Question: Why are we using this materials in the experiment is there any reason why we using it? Quantification of Total Protein in Serum Experiment; Materials: Fatty acid free beef albuminl solution: 100 g / L Reagent Ⅰ Sodium potassium tartrate 9g / L Sodium hydroxide 0.2 mol / L Potassium iodide 5 g / L Reagent Ⅱ Serum from blood Copper sulphate solution: 150 g / L Working solution: 5 ml of reagent copper sulphate solution is prepared by mixing into 1 L of reagent Ⅱ. This reagent is stable for 6 months at 2-8 ° C Procedure: *Three test tubes are taken. These are marked as blank, standard and sample tubes. *Only 5 ml of working solution is put into the blank tube. *0.1 ml reagent I and 5 ml working solution are put into the standard tube. *0.1 ml of serum and 5 ml of working solution are put into the sample tube. *After mixing each tube separately, it is waited for 30 minutes at room temperature and measured at 545 nm in the spectrophotometer. *Color permanence is 30 minutes
Question: Why are we using this materials in the experiment is there any reason why we using it? Quantification of Total Protein in Serum Experiment; Materials: Fatty acid free beef albuminl solution: 100 g / L Reagent Ⅰ Sodium potassium tartrate 9g / L Sodium hydroxide 0.2 mol / L Potassium iodide 5 g / L Reagent Ⅱ Serum from blood Copper sulphate solution: 150 g / L Working solution: 5 ml of reagent copper sulphate solution is prepared by mixing into 1 L of reagent Ⅱ. This reagent is stable for 6 months at 2-8 ° C Procedure: *Three test tubes are taken. These are marked as blank, standard and sample tubes. *Only 5 ml of working solution is put into the blank tube. *0.1 ml reagent I and 5 ml working solution are put into the standard tube. *0.1 ml of serum and 5 ml of working solution are put into the sample tube. *After mixing each tube separately, it is waited for 30 minutes at room temperature and measured at 545 nm in the spectrophotometer. *Color permanence is 30 minutes
Chemical Principles in the Laboratory
11th Edition
ISBN:9781305264434
Author:Emil Slowinski, Wayne C. Wolsey, Robert Rossi
Publisher:Emil Slowinski, Wayne C. Wolsey, Robert Rossi
Chapter43: Analysis For Vitamin C
Section: Chapter Questions
Problem 3ASA
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Question: Why are we using this materials in the experiment is there any reason why we using it?
Quantification of Total Protein in Serum Experiment;
Materials:
Fatty acid free beef albuminl solution: 100 g / L | Reagent Ⅰ |
Sodium potassium tartrate 9g / L Sodium hydroxide 0.2 mol / L Potassium iodide 5 g / L |
Reagent Ⅱ |
Serum from blood | |
Copper sulphate solution: 150 g / L | |
Working solution: 5 ml of reagent copper sulphate solution is prepared by mixing into 1 L of reagent Ⅱ. This reagent is stable for 6 months at 2-8 ° C |
Procedure:
*Three test tubes are taken. These are marked as blank, standard and sample tubes.
*Only 5 ml of working solution is put into the blank tube.
*0.1 ml reagent I and 5 ml working solution are put into the standard tube.
*0.1 ml of serum and 5 ml of working solution are put into the sample tube.
*After mixing each tube separately, it is waited for 30 minutes at room temperature and measured at 545 nm in the spectrophotometer.
*Color permanence is 30 minutes.
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