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Question 10
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- Quèstion 34 Enzyme that joins the DNA fragments O Recombinant DNA Technology O Restriction enzymes O Ligase O Palindromic sequencesQuestion 11 Each cycle of amplification in PCR involves all of the steps EXCEPT: A) annealing of oligodeoxyribonucleotide primers to DNA. B) the addition of fresh dNTPs to the reaction mixture. C reaction with DNA polymerase at approximately 70°C. D) thermal denaturation of the target duplex DNA.Question 6 DNA ligases will seal all nicks there are in the DNA strands during the replication process and in DNA repair. A) True (B) False
- Question 49 The Sanger method of DNA sequencing follows the principle of complementarity just like in the replication process. A) True B) FalseQuestion 3. Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. a) Polymerase chain reaction (PCR) is an artificial method to amplify DNA segment into thousand copies of that DNA. i. Elaborate the three steps in PCR which based on temperature manipulation in controlling the replication process. Distinguish the uniqeness of Reverse Transcriptase PCR (RT-PCR) technique as ii. compared to the common PCR. iii. Relate the application of RT-PCR and Rapid Diagnostic Test for biomarker detection with the typical time course of COVID-19 infection.Question 17 What is the nucleotide sequence of the DNA strand that is complementary to 5'-ATCGCAACTGTCACTA-3 A 5'-UAGUGACAGUUGCGAU-3' B 5-TAGCGTTGACAGTGATA-3 5'-ATCACTGTCAACGCTA-3 D 5'-TAGTGACAGTTGCGAT-3'
- Question 10 When DNA replication occurs in eukaryotic cells before Mitosis or Meiosis, the DNA starts to separate at multiple origins of replication. As the DNA continues to separate, a is formed. Question 10 options: A) B) C) D) DNA Bubble DNA Fork Daughter Cell Replication BubbleQuestion 28 Intrinsic RNA chain termination is determined by specific sequences in the DNA called sites. A supercoil B termination rho binding D template bindingQuestion 3 Bacteria defend against phages by Natural selection favors mutation can not be recognized by virus. O all of these except DNA replication O restriction enzymes CRISPR-Cas system O DNA replication
- Question 5 DNA polymerase: |A Is able to open the helix at the origin of replication. B Works on both strands during replication. C Synthesizes phosphodiester bonds between the two strands of the DNA helix. D] Is an enzyme that recognizes the gene pro- moter. E Is responsible for the transcription.Question 5 Okazaki fragments are short DNA pieces that explain how the DNA polymerase can continue the synthesis of the new strand. A) True B) FalseQuestion 1. Enzymes, proteins and deoxyribonucleic acid (DNA) macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA repreduction. are important biological a) Compare the process of protein synthesis between eukaryote mRNA and viral RNA b) With the aid of a diagram, draw an adapter molecule that recognizes the codons of mRNA and explain its functions in DNA translation.