Provided with the following data, compute the corresponding CFU/ml of the original culture. ssume that spread plate technique was done. Show your solutions. 10-1 10-2 10-3 10-4 10-5 Plate 1 TNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 TNTC 280 90 22 2
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- With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?Provided with the following data, compute the corresponding CFU/ml of the original culture. Assume that spread plate technique was done. Show your solutions. 10-1 10-2 10-3 10-4 10-5 Plate 1 TNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 TNTC 280 90 22 2A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
- Below is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uLt/Root/Pages/TestTaker/MonitorLaunch.aspx?id%3DE9B80AE3-962E-4628-B79D-A3F06F1CAB72 MonitorLaunchaspx?id%3DE9B80AE3-962E-4628-879D-A3F0EFICABT2-Google Chrone uninoculated tube The medium shown here was inoculated with a bacterium then incubated for 48 hours; an uninoculated control tube is also present for comparison. This particular bacterium gave a for this test. a) O negative result b) O positive result c) O indeterminate result Review Later d) O variable resultPrepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:
- In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. a. What is the total volume of the medium needed? b. What is the amount of each component needed, given the following media composition per liter of distilled water:Design a serial dilution of a 25-gram cheese sample to achieve a final dilution of 10-5 using 90 ml blanks. Show how you would plate out 10-2 through to 10-6
- A laboratory technician performed a series of 10 fold serial dilutions shown below. he plated 0.1 mL from each dilution tube onto NA and got the follow numbers per plate dilution 1:10 1:10 1:100 1:1000 1:10000 1:100000 colony count >1000 >1000 808 303 38 3 calculate the concentration of bacteria in the undiluted stock (in CFU/mL)If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original culture which had been diluted 1x10^-4 times, what is the number of CFU/ml of the original culture?First There Was One, Then There Were Two RESULTS Results of the plate counts for the samples you worked with. Number of Colonies for Each Dilution Plated Time Sampled 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10:40 TNTC TNTC TNTC 280 TFTC TFTC3 T FIC2 TFTC TFTC 13 TNTC TNTU TNTC TNTC I60 TNTC TNTC 1:00 TFTC q a TFTC O 1:20 11:40 12:00 TNTC TNTC87 TFTC TFTC, TFICg TNTC ITNTC ITNTC TNTC 125 TNTC TNTC TNTC TNTC 164 TFIC 13 T+TC Calculate the cfu/ml for each time sample you worked with in the space below and record those values in the table below.