Procedure A Take 3 ml Add 50 ul of Top up to 50ml. - Add fresh LB Mix Sml ampicillin to the overnight culture- mixture to mixture with 45 ml LB analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 Overnight culture - incubated at shaked incubator at 37°c 200rpm - analyse (OD600)record as zero time-point. mins. Stop until (E-coli + LB borth) · 75 mins. Mix overnight culture 5ml Take 3 ml Add 50 ul of ampicillin to mixture No ampicilin mixture analyse OD600 to the B (control) with 45 ml LB broth the Withdraw 3ml mixture to analyse (OD600)record as zero time-point. The mixture is then Measure every 15 mins. Stop until incubated at shaked incubator at 37°c 200rpm 75 mins. Result Group 1 Group 2 Group 3 OD 600 (A) (B) (A) (B) (A) (B) Time (minutes) Ampicilin Control Ampicilin Control Ampicilin Control 0.124 0.248 0.315 0.289 0.133 0.121 15 0.756 0.367 0.485 0.162 0.168 0.167 30 0.645 0.428 0.757 0.057 0.248 0.053 45 0.633 0.695 1.025 0.052 0.339 0.018 60 0.599 0.937 1.434 0.05 0.509 0.053 75 0.593 1.301 1.778 0.039 0.676 0.053
Procedure A Take 3 ml Add 50 ul of Top up to 50ml. - Add fresh LB Mix Sml ampicillin to the overnight culture- mixture to mixture with 45 ml LB analyse the both broth OD600 The mixture is then Withdraw 3ml mixture to Measure every 15 Overnight culture - incubated at shaked incubator at 37°c 200rpm - analyse (OD600)record as zero time-point. mins. Stop until (E-coli + LB borth) · 75 mins. Mix overnight culture 5ml Take 3 ml Add 50 ul of ampicillin to mixture No ampicilin mixture analyse OD600 to the B (control) with 45 ml LB broth the Withdraw 3ml mixture to analyse (OD600)record as zero time-point. The mixture is then Measure every 15 mins. Stop until incubated at shaked incubator at 37°c 200rpm 75 mins. Result Group 1 Group 2 Group 3 OD 600 (A) (B) (A) (B) (A) (B) Time (minutes) Ampicilin Control Ampicilin Control Ampicilin Control 0.124 0.248 0.315 0.289 0.133 0.121 15 0.756 0.367 0.485 0.162 0.168 0.167 30 0.645 0.428 0.757 0.057 0.248 0.053 45 0.633 0.695 1.025 0.052 0.339 0.018 60 0.599 0.937 1.434 0.05 0.509 0.053 75 0.593 1.301 1.778 0.039 0.676 0.053
Chapter15: Antibiotic Agents
Section: Chapter Questions
Problem 25RQ
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