Procedure 1. Place 2mL of milk in each of the 3 test tubes. 2. Heat one of the test tubes to boiling and cool. 3. Add 5 drops of 1% Methylene blue solution to each test tube. 4. Add 2 drops of 1% Formaldehyde to the test tube containing boiled milk and to the other containing unboiled milk leaving the third tube as a control. 5. Mix well. 6. Add 1mL of Paraffin oil to each test tube and warm in a water bath for 10-15 minutes at 40°C. 7. Observe the change that is taking place in each test tube.
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Experiment: Animal Oxidase
Procedures are indicated below. (Please view the image)
Question: What will be the possible result and observation? What's the principle behind the results?
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- Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.1. What happens if sample for Potassium determination is refrigerated? What causes the variation? 2. What is the role/importance of Potassium in the body metabolism? 3. In doing the assay, why is hemolyzed sample not allowed?Tube Observation Inference 0.1000 M glucose Red precipitate 0.0500 M glucose Red precipitate 0.0250 M glucose Red precipitate 0.0100 M glucose Green precipitate 0.0010 M glucose Green precipitate 0.0001 M glucose Blue solution 0.1000 M lactose Red precipitate 0.1000 M sucrose Blue solution 0.1000 M fructose Red precipitate Urine 1 Green solution Urine 2 Orange precipitate Table 3. Results of Benedicts Test on different samples
- 1. Place 5 mL of starch solution in the test tubes. 2. Heat the test tubes to boiling and add to 1 mL of saliva, Cool and then continue heating but keep the test tube in a water bath at temperature of 40oC 3. At 5 minutes interval for 30 minutes take a drop of the reaction mixture from each test tube and test with Iodine solution (use a spot plate for the test and stir the contents of the test tube before taking a drop). Tabulate the results. 4. What would be the color of saliva extract with iodine in 3, 6, 9,12,15,and 18 minutesProcedure:1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva toeach tube. Mix thoroughly.2. Place the first tube in ice water, the 2ndtube leave at room temperature, the 3rdtube in 40°C , the 4thtube at 60°Cwater bath and the 5thtube boil for 2minutes..3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4thtubeallows to stand for 30 minutes after heating for 2 minutes.4. Test the contents of each tube with iodine and benedict’s tests.1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to completely grind the beef? 3. Why is necessary to balance the homogenate tubes for centrifugation? 4. How do you prepare a 100mL of 0.1 M phosphate buffer? 5. From the anterior buffer, how do you make 100mL of 0.05 M? 6. Calculate the amount of ammonium sulfate necessary to get a 20% solution? 7. Which is the importance of dialysis?
- Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.1. Give the importance and purpose of the Cetrimide Test. 2. Discuss briefly how each of the different oxidase method is being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.1&3/4 tbs po BID x 7 days #QS How many milliliters should the pharmacy dispense?
- 2. Addition of a small amount of strong acid or strong base to a buffer: effect on pH 2. Add 5 drops of lacmoid indicator solution to each test tube and mix the content. Record the color.II. Tests for Lipids A. Bromine Test Table 3. Results for Bromine test. SAMPLE Ranking of the number of drops of Bromine Degree of unsaturation Oleic oil Coconut oil Soybean oil * Rank (1", 2nd, 3rd) – 1ª as the highest number of drops; 3d as the lowest number of drops ** minimal, moderate, highest B. Acrolein Test Table 4. Results for the Acrolein test. INFERENCE** SAMPLE Oleic oil Coconut oil Beef fat Egg yolk Pungent odor or no observable result * positive or negative for glycerol OBSERVATION c. Zak's Test Table 5. Results for Zak's test. SAMPLE OBSERVATION INFERENCE*. Oleic oil Coconut oil Egg yolk * Purple product or no observable result * positive or negative for cholesterol D. Saponification Table 6. Results for saponification. SAMPLE REMARKS* Soap suspension (Step 2) Purified soap (Step 3) Acid hydrolysis product of soap (Step 4) Rank (14, 2nd, 3rd) as to the estimated pH value -1 as the highest pH value; 3rd as the lowest pH value1. Fill up the table below to summarize the confirmatory test for casein and whey. Test Sample Filtrate Residue / Precipitate Milk Component Reagents Observations Inferences 2. Test for Milk Fat Briefly describe how milk fat is being tested?