Primer 1 primes synthesis of the 5' to 3' strand (i.e. sense strand) of an oat cDNA/gene and encodes for first few amino acids appearing at the N-terminal end of the corresponding polypeptide. Primer 156 primes synthesis of the 3' to 5' strand (i.e. antisense strand) of an oat cDNA/gene and encodes for the last few amino acids appearing at the C-terminal end of the polypeptide. The polypeptide encoded by this gene is 156 amino acids in length. QUESTION:-Assume that a PCR using the combination of Primer 1 and Primer 156 plus oat genomic DNA resulted in the amplification of a DNA fragment of the 160bp larger than PCRs in (1) above. With respect to the structure to this relevant gene, what does this result indicate? Explain how you reached this conclusion
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Primer 1 primes synthesis of the 5' to 3' strand (i.e. sense strand) of an oat cDNA/gene and encodes for first few amino acids appearing at the N-terminal end of the corresponding polypeptide. Primer 156 primes synthesis of the 3' to 5' strand (i.e. antisense strand) of an oat cDNA/gene and encodes for the last few amino acids appearing at the C-terminal end of the polypeptide. The polypeptide encoded by this gene is 156 amino acids in length.
QUESTION:-Assume that a PCR using the combination of Primer 1 and Primer 156 plus oat genomic DNA resulted in the amplification of a DNA fragment of the 160bp larger than PCRs in (1) above. With respect to the structure to this relevant gene, what does this result indicate? Explain how you reached this conclusion
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