position of the Start and Stop. The GFP and RFP genes shown do not have start

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Cre-Lox system is used for site-specific modification of DNA for genetic engineering applications. The reporter gene construct
shown below is used to test the Cre-Lox system. Cells are transfected with the construct shown below and the activity of the
constructs is determined by visualizing the cells with a fluorescence microscope. Match the following conditions with the expected
cell observations. Hint: Make sure you note the position of the Start and Stop. The GFP and RFP genes shown do not have start
codons.
Cre/Lox Reporter Gene
ATG
LoxP
CMV-Pro
A. No Expression
GFP
Stop
B. GFP Only
LoxP
Absence of Cre
Cells treated with drug that induces expression of Cre
RFP
C. RFP Only
A. Image A
B. Image B
C. Image C
D Image D
express
?
D. RFP and GFP
Transcribed Image Text:Cre-Lox system is used for site-specific modification of DNA for genetic engineering applications. The reporter gene construct shown below is used to test the Cre-Lox system. Cells are transfected with the construct shown below and the activity of the constructs is determined by visualizing the cells with a fluorescence microscope. Match the following conditions with the expected cell observations. Hint: Make sure you note the position of the Start and Stop. The GFP and RFP genes shown do not have start codons. Cre/Lox Reporter Gene ATG LoxP CMV-Pro A. No Expression GFP Stop B. GFP Only LoxP Absence of Cre Cells treated with drug that induces expression of Cre RFP C. RFP Only A. Image A B. Image B C. Image C D Image D express ? D. RFP and GFP
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