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- Q4 (a) Analyze the importance of dropping mercury electrode on polarography. (b) (i) Calculate the molarity of MgSO4 in a solution containing 1.5 g ofMgSO4 in a volume of 250 mL?(ii) Calculate the weight of MgSO4 in grams which is present in 100mL of 0.076 M MgSO4?(c) Evaluate the key differences between paper chromatography and gaschromatography(a) You measure the percent transmittance of a solution containing chromophore X at 400nm in a 1-cm path length cuvette and find it to be 50%. What is the absorbance of this solution? (b) What is the molar absorptivity of chromophore X if the concentration of X in the solution measured in question (a) is 0.5mM? (c) What is the concentration range of chromophore X that can be assayed if, when using a sample cell of path length 1, you are required to keep the aborbance between 0.2 and 0.8?The following data obtained compounds separated on a 20m capillary tube. What is the column length required for a resolution of 1.5?
- A molecular exclusion column has a diameter of 7.8 mm and a length of 30 cm. The solid portion of the particles occupies 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polymers of various molecular masses is eluted between 23 and 27 mL. What does this imply about the retention mechanism for these solutes on the column?cont irmation True or false: The purpose of the internal standard is to make quantitation of the analyte insensitive to injection errorsWhat are the similarities between fluorescence detectors in HPLC and chemiluminescence detectors in HPLC?
- (b) For column 1(i) What are the unadjusted retention times for components A and B?What major impact does reducing film thickness of stationary phase in a wall-coated capillary column have on the separation efficiency (i.e., band broadening) in GC: Shorter tr (retention time) Lower C-term Lower resolution Higher A-term Higher B-termA method of quantitative determination of the concentration of constituentsof a sample analyzed by gas chromatography is area normalization. Thiscomplete elution of all sample constituents is required. Thearea of each peak is measured and corrected for the detector response to the diffe- renteseluatos. This correction involves dividing the area by an empiricalcorrection factorDetermined. The concen ration of the analyte is found from the ratio between itscorrected and the total corrected area of all peaks. For a chromatogram containing threerelative areas were determined as 16.4; 45,2; and 30.2, in the order ofincreased retention time. Calculate the percentage of each compound if therelative responses from the detector are 0.65, 0.73 and 0.85, respectively.
- Why does the efficiency (decreased plate height) of liquid chromatography increase as the stationary phase particle size is reduced?What could be the effect of trapped bubbles in the viscometer during the run on (a) the measured time? (b) the relative viscosity? Explain your answers.In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller molecules