o determine the pKa of PNP, a student needs to prepare a solution of 18.0 µM PNP in 26.0 mM MES pH 6.3, with a total volume of 950 µL. Stock solutions of 0.350 mM PNP and 100.0 mM MES pH 6.3 are available. Calculate the volume of PNP required to make the solution (in µL).

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
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Chapter17: Complexation And Precipitation Reactions And Titrations
Section: Chapter Questions
Problem 17.24QAP
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To determine the pKa of PNP, a student needs to prepare a solution of 18.0 µM PNP in 26.0 mM MES pH 6.3, with a total volume of 950 µL.  Stock solutions of 0.350 mM PNP and 100.0 mM MES pH 6.3 are available.  Calculate the volume of PNP required to make the solution (in µL).

 
 
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