[methyl-"C]Pyruvate was administered to isolated liver cells in the presence of sufficient malonate to block succinate dehydrogenase completely. After a time, isocitrate was isolated and found to contain label in both carbon 2 and carbon 5: 14CH,-Co0- HC-CO0 OH How do you explain this result?

[methyl-"C]Pyruvate was administered to isolated liver cells in the presence of sufficient malonate to block succinate dehydrogenase completely. After a time, isocitrate was isolated and found to contain label in both carbon 2 and carbon 5: 14CH,-Co0- HC-CO0 OH How do you explain this result?

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter23: Enzymes

Section: Chapter Questions

Problem 23.18P: 8 The optimal temperature for the action of lactate dehydrogenase is 36°C. It is irreversibly...

See similar textbooks

Related questions

Q: Match the following enzymes in Rows with their classification in Columns. sucrase phosphokinase…

A: Examples of various types of enzymes

Q: What is the difference between the whole Aspartate Transcarbamoylase (ATCase) enzyme and an ATCase…

A: Allosteric enzymes are enzymes that change their conformational ensemble upon binding of an…

Q: :B H-O R, HO HO H RC-C RC-cH ŚCOA ŚCOA ŠCOA 15. Explain which class of enzyme being represented. The…

A: This is an example of fatty acid metabolism in which fats are converted into energy by the formation…

Q: 9. (а) in-line Provide an example of an displacement mechanism' to depict the chemical/biochemical…

A: In the chemical/biochemical interaction of D-glucose with ATP the reaction is catalyzed by EcAGPase…

Q: 4. What allosteric effector below is NOT involved in the regulation of phosphorylas A. insulin B.…

A: Given: allosteric effectors. To find: allosteric effector not involved in the regulation of…

Q: Arsenate (AsO43-) has been used as a poison since ancient times. It disturbs glycolysis by…

A: Glycolysis is the splitting of glucose molecule into pyruvate. This process takes place in the cell…

Q: OPO,2- \OH HO myo-inositol-1-phosphate synthase OH 2-03PO HO "OH HO (1 ÕH a-D-glucose-6-phosphate…

A: Absolute configuration is R. This can be calculated based on the CIP rule.

Q: Analyze each reaction by considering the functional groups that change, the coenzyme or other…

A: To find: Analysis of the given reaction by considering the functional groups that change, the…

Q: >Write all the steps in the transamination reaction of PMP with a -ketoglutarate plus a lysine…

Q: a. Identify the molecules. Which molecule tends to be catalyzed by a lipase? Justify. b. Change or…

A: Lipase is an enzyme that mainly helps to digest the dietary fats and it is produced by the pancreas.…

Q: 4. The AG for the aldolase reaction is 22.8 kJ/mol. When the reaction is run in a hepatocyte cell at…

A: Given that, the aldolase reaction of fructose 1,6-bisphosphate to glyceraldehyde 3-phosphate and…

Q: Aldolase shows no activity if it is incubated with iodoacetic acid before fructose-1,6-bisphosphate…

A: Thiol is a good nucleophile whereas iodine is considered as a good leaving group. So the residues of…

Q: FAD is covalently bound to succinate dehydrogenase (the enzyme whose mechanism was shown on the…

A: FAD is covalently bound to succinate dehydrogenase as a result of a base removing a proton from C-8…

Q: Given the following information on reduction potentials, calculate the standard free energy in…

Q: What class of enzyme catalyzes each of the following reactions? Hydrolysis of Val-Gly to Val and…

A: Enzyme: The proteins that helps chemical reaction to occur within the cells by increasing the rate.

Q: 23. Enzyme + acetate + CoA -> enzyme + acetyl–CoA In the reaction above, the enzyme is acting as…

A: A catalyst catalyse a reaction without being consumed in the reaction.

Q: SHOW/IDENTIFY the arrow pushing mechanism in at least 4 steps. CH,OPO- c=0 | 2 HO-CH, CH,OPO- | 1…

A: The objective of the question is to show the arrow pushing mechanism in at least 4 steps .

Q: 4. Lactate dehydrogenase has a 1000-fold selectivity for lactate as a substrate over malate.…

A: The substance which enhances the rate of biological processes is called enzymes. Enzymes are…

Q: 6. The AG of the citric acid cycle reaction catalyzed by succinyl-CoA synthetase is approximately 0…

A: Citric acid cycle is the metabolic pathway for the oxidation of various intermediate molecules such…

Q: Part c

A: The ∆G0 for the conversion of X into Y is 20 kJmol-1. Since one kilojoule is equal to thousand…

Q: Deamination reactions typically involve which of the following (select all that apply)? O glutamate…

A: Amino acid glutamate is involved throughout much of the oxidative deamination that occurs in cells,…

Q: Suppose you added a "pulse" of 3-14C-pyruvate (where 14C is in the methyl position) to an…

A: a. See the picture(the leveled carbon is shown in a circle) . If pyruvate is leveled with 14C at the…

Q: In glycolysis, why must glucose-6-phosphate isomerize to fructose-6-phosphate before the cleavage…

A: Glycolysis is the linear sequence of the reactions by which glucose is enzymatically split into two…

Q: Match the coenzymes and/or substrates with their corresponding glycolytic enzymes. Note that an…

A: Here the coenzyme or substrates are ATP, NAD+,Dihydroxy acetone phosphate and their glycolytic…

Q: Rh() -CH, + H, - C,H, AG =-150.97 kJ/mol L CH,0, - C,H,0, + H,0 AG = 3.1 kJ/mol O C,H,,0,P + ATP %3D…

Q: 23.Carbamyl phosphate synthetase_is a mitochondrial enzyme; it uses ammonium as the source of…

A: 23. correct option : A. 1, 2 Carbamyl phosphate synthetase 1 is a mitochondrial enzyme and uses…

Q: Part b

A: (b)The ∆G○ for hydrolysis of ATP is -30.5 kJ/mol. The G○rxn for the overall reaction is calculated…

Q: C,H1206 (aq) [C,H1005]n (aq) + H20(1) а-amylase [C,H1005]n-1 (aq) + (glucose) (starch)

A: Given: The reaction, C6H10O5naq+H2Ol→α-amylaseC6H10O5n-1 aq+C6H12O6aq.Starch…

Q: An experimenter using acetyl-CoA with a 14C on the carbonyl group traces the radioactive label in…

A: A) No carbons of cholesterol will be labeled if mevalonate labeled with 14C in the carboxyl carbon…

Q: Which of the following would occur in a mutant lacking Ferredoxin-thioredoxin reductase? (a).…

A: In this question, we have to find out the correct answer of given problem by the help of the mutant…

Q: 4. Pyridoxal Phosphate Reaction Mechanisms Threonine can be broken down by the enzyme threonine…

A: THREONINE DEHYDRATASE: It is also known as threonine deaminase. It is an enzyme which is used in…

Q: |Circle the most acidic hydrogen on each molecule used in the acetate pathway: of H SCOA На- SCOA…

Q: ydrogen per Exide in the pr hydroxide Deroxidase

A: Part 1: We know that "Uricase acts on uric acid to produce allantoin, carbon dioxide and hydrogen…

Q: Which steps below require an ATP to run? O Phosphorylation Isomerization O Second Phospnorylation…

A: ATP is required in phosphorylation steps . ATP ----> adinosine triphosphate . It gives phosphorus…

Q: 1-NADH dehydrogenase doesn't contain : A.Several FMN and Fe-S centers B. a binding site NADH C.…

A: BADH dehydrogenase is a Flavoprotein. Contains Fe - S clusters. It is the first large protein…

Q: can you tell me step by step, what is happening to convert L-luciferin to D-luciferin? the chemistru…

A: The chemical compound that is responsible for the bioluminescence of certain living organisms is…

Q: 2. Using the structure of glycogen shown here (or any other source material), answer the following…

A: Given Enzyme = Glycogen Phosphorylase

Q: Triose phosphate isomerase catalyzes the conversion of dihydroxyacetone phosphate (DHAP) into…

A: The reactant is dihydroxyacetone phosphate (DHAP). The product is glyceraldehyde-3-phosphate (G3P).…

Q: OPO 2- 2-03PO HO myo-inositol-1-phosphate synthase HO HO HO HO" "OH (1) ÕH a-D-glucose-6-phosphate…

Q: Using the AGO values found in your Biochemistry textbook, what metabolite concentrations would you…

A: Since you have asked a question with multiple subparts, we will answer only first 3 subparts for…

Q: What acyl group does pyruvate decarboxylase transfer to a proton? a. What acyl group does the…

A: The pyruvate decarboxylase is enzyme used to transfer acyl group from one group to another group…

Q: Carbonic anhydrase is an enzyme that catalyzes the conversion of carbon dioxide to bicarbonate ion…

A: Carbonic anhydrase is an enzyme establish in the red blood cells. It catalyzes the reaction CO2 and…

Q: Identify the lettered intermediates in the following reaction scheme. Thisis an alternative method…

A: The intermediates obtained in Gabriel synthesis is given below:

Q: 4. A flavin dependent monooxygenase can degrade 4-chlorophenol to benzoquinone. Propose a mechanism…

A: While explaining a mechanism of a reaction, the movement of electrons is shown by curved arrows. The…

Q: Predict the product@ and propase a mechamism for the following B-eliminahin reachin. If there is…

Q: Given the following information on reduction potentials, calculate the standard free energy in…

A: Given: Lipoamide + 2H+ + 2e-→ dihydrolipoamide, Eο = -0.29 V FAD + 2H+ + 2e- → FADH2, Eο = −0.01 V

Q: . Can the reaction (a) be driven by coupling to the hydrolysis of ATP (b)? The AGO value in kJ/mol…

A: The reaction occurs spontaneously when the value of change in the Standard Gibbs free energy is…

Q: 2. Using arrow formalism, draw the reaction mechanism for the steps of glycolysis specified below.…

Q: important to the process of the complex

A: In this question we have to explain that why is the reaction that is catalyzed by E3 in the…

Q: In ATP synthase, which subunit helps to prevent a and 3 sub units from rotating as protons move…

A: The F0 region of ATP synthase consists of three main subunits a, b, and c. Six c subunits make up…

Question

[methyl-"C]Pyruvate was administered to isolated liver cells in the presence
of sufficient malonate to block succinate dehydrogenase completely. After a
time, isocitrate was isolated and found to contain label in both carbon 2 and
carbon 5:
14CH,-Co0-
HC-CO0
OH
How do you explain this result?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Similar questions

24-22 How is the catalytic unit of protein kinase activated in adrenergic neurotransmission?
How would you classify the enzyme that catalyzes the following reaction? -OOC. H Transferase Fumarate Hydrolase Ligase Isomerase Oxidoreductase H Lyase COO H₂O fumarase HRII!!!!! Hs OOC S-malate COO- OH
a Lactate dehydrogenase H H3C-C-coO- ОН ooc-C-CH3 L-(+)-Lactase Pyruvate This is a(an) b Creatine kinase CH3 O CH3 O H2N, АТР ADP NH2 NH2 Adenosine triphosphate Adenosine diphosphate Creatine Phosphocreatine This is a(an)
wtgers Whch y hde in pntace to ac the futs by the Syt mechariount 5888
Q11: For the following enzyme catalyzed reaction, indicate where the transition state occurs and whether you expect the enzyme to increase the transition towards the product? Product Reactant Reaction Progression Q12: For the following endergonic reaction, would you expect the presence of malate dehydrogenase to facilitate this reaction proceeding towards oxaloacetate, when both malate and oxaloacetate are present in equimolar amounts? ÇOO- fo0 NAD* COO- NADH + H* Но—с—Н 0=C CH2 CH2 malate CO" COO- dehydrogenase L-Malate Oxaloacetate AG'° = 29.7 kJ/mol AG Free Energy
The enzyme that catalyzes reaction below can be classified as: NAD* NADH + H* Но- H-Ć- Н—с—н H- malate dehydrogenase Oxaloacetate Malate
The second step in the catabolism of most amino acids is the removal of the nitrogen atom from glutamate by oxidative deamination. In this reaction, glutamate is converted to an a-keto acid. Modify the structure to show the a-keto acid product. ?- || с I CH₂ I H₂N- & + remaining products enzyme CH₂ I CH₂ + NAD+ + H₂O CH₂ Incorrect Enter the chemical formulas for the remaining products. remaining products: NADH + H+ + NH Identify the enzyme that catalyzes the reaction. The enzyme that catalyzes the reaction is glutamate dehydrogenase. OH OH
Which of the following enzymes catalyzes a reaction with the pictured compound as an intermediate? = Hz C—6 ཆོས་རིག་ག་ག་ག་ག་ག་གས་པ་ 0—P, O succinyl-CoA synthetase fumarase succinate dehydrogenase malate dehydrogenase a-ketoglutarate dehydrogenase
Oxaloacetate is an inhibitor of succinate dehydrogenase because it is structurally very similar to succinate as shown below. Also, its binding to the enzyme does not involve any covalent bond formation. What type of inhibitor is oxaloacetate? COO | CH₂ 1 CH₂ 1 COO Succinate COO | CH₂ C=O COO Oxaloacetate O a noncompetitive inhibitor both a noncompetitive and an irreversible inhibitor O an irreversible inhibitor O a competitive inhibitor
Which coenzyme is responsible for the following reaction? CO,H CO,H OH HO.. ОН ОН ОН O TPP (thiamine pyrophosphate) O NAD* (or NADP*) Ο ΚΗ2 O FADH2 В12 O PLP (pyridoxal phosphate) O one of the THE (tetrahydrofolate) coenzymes O NADH (or NADPH) O biotin O FAD
Draw the product formed by the reaction of acetyl CoA with glutamate in a reaction catalyzed by N-acetylglutamate synthase. Use wedges and dashes to indicate the stereochemistry at asymmetric centers. Provide the structure in the protonation state found in physiological conditions. COO™ H&N | N-acetylglutamate synthase SCOA COO™ [] Q
Summerize importan functions of Glutathione Reductase. Where it is made? In all cells or only specific organs/ tissues?