look at the result of the following genotyping of three different members of the same family the to bonds in the gel electrophoresis result of the daughter suggest dna products of different lengths what does it mean
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- An Hfrstrain that is a *b*c*d* e*f* g *h* is mated with an F strain that is a b e d e f gh. The mating is interrupted at 5 minutes interval, and the genotypes of the F recombinants are determined. The results obtained are tabulated in Table 2. Draw the map of the Hfrchromosome and indicate the position of the origin of transfer, the direction of the transfer and the minutes between genes. Table 2:Entry time of Hfr chromosome into recipient cell. Time a d e f h 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +A molecular biologist is investigating homologous recombination. One aim of this study is to reconstitute stages of the process in vitro. Draw diagrams to show how the four synthetic oligonucleotides below could base-pair to form a stable model Holliday junction. W 5’ GATCGCATTGTAGCCGTAGGTCCACTGTAA 3’ X 5’ GTCCCATACGTAGCCGTAGGACATGTACCG 3’ Y 5’ CGGTACATGTCCTACGGCTACAATGCGATC 3’ Z 5’ TTACAGTGGACCTACGGCTACGTATGGGAC 3’Describe the Holliday model and the double-strand break model for homologous recombination
- In an electrophoretic gel across which is applied a powerful electrical alternating pulsed field, the DNA of the haploid fungus Neurospora crassa (n = 7) moves slowly but eventually forms seven bands, which represent DNA fractions that are of different sizes and hence have moved at different speeds. These bands are presumed to be the seven chromosomes. How would you show which band corresponds to which chromosome?Post replicative recombination between homologous chromosomes has 2 major benefits. What are theyCONNECT Two loci exhibit 5% recombination between them. How many map units apart are they?
- The results of a paternity test are shown in the table below. Numbers indicate the number of short tandem repeats for loci tested. Whos the daddy? How sure are you?Tick all the essential steps to demonstrate a genetic linkage between a disease and a molecular marker in humans. identify the alleles of the genetic marker only for diseased individuals in the pedigree enumerate parental type individuals sequence the wild-type and mutant alleles to find the mutation no correct answer calculate a Lod score calculate the recombination frequency between the mutation and the molecular marker identify the alleles of the genetic marker for each individual in the pedigree pedigree analysis cloning the defective gene enumerate recombinant individualsFor each genotype in the table below, determine whether or not functional B-gal will be produced in the presence or absence of the inducer. Write a plus (+) if B-gal is produced or a minus (-) if it is not. ma Chromosome F' lac (plasmid) - Inducer + Inducer ---- --- --- I*O*Z I*O°Z+ I*O*Z* I* = wildtype repressor |- - no functional repressor produced O* - wildtype operator OC = operator mutation prevents repressor from binding %3D
- The following recombinants are recovered when conjugation occurs between an a*d*g+ donor and an adg recipient. at d+ g+ = 84% a d g+ = 6% at d g+ = 10% a dt g+ = less than 1% What is the map distance between the a and d genes? 10 map units 74 map units less than 1 map unit 84 map units 6 map unitsHere is a strawberry karyotype - ignore the different colours. How many chromosomes are shown in the figure? What is the ploidy of this type of strawberry? How many alleles could an individual strawberry have at each locus? Finally during the G2 stage of cell division, how many double helixes would be in this picture if you drew them over the chromosomes? Many domesticated plants are polyploid - give one possible reason why this might be the case, with a focus on using genetic principles to reason it out (i.e., you don't have to be right per se, you have to build an argument based on what you've already learned).Shown below are eight DNA sequences from different individuals QHow many different haplotypes (sets of linked variants) are found in these eight sequences
