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- What is the difference between a myofilament and a myofibril?Define myofilamentExperimenters can separate F-actin thin myofilaments frommyosin thick myofilaments. First they homogenize musclecells in a blender (to break cell membranes); then they place thehomogenate in a Ca2+-free “relaxing solution” that contains ATP.Explain why ATP must be present and Ca2+ ions must not bepresent in order to isolate thick and thin myofilaments from eachother.