Hello, can someone please help me? I have already hand graphed 1 and 2 and found the v max, however I cannot find the Km value. I am confused because the plots on the linear weaver burk plot do not extend past the values that make up the line. So how am I supposed to estimate the value for the y-intercept in order to find the Km value Lisa is running an experiment to determine the kinetic parameters (Km and Vmax) ofthe enzyme that she is studying. She has run a series of experiments where shechanged the amount of substrate added and measured the accumulation of productover a 5 second time interval. She used this data to calculate the dependence of therate of the reaction on substrate concentration. Her results listed below.SubstrateConcentration (mM)Velocity (mM/sec)2.546.37.611020You need to hand-1. Using this information construct a Michaelis-Menten curve.draw on graph2. Using this information construct a Lineweaver-Burk plot.paper (no excel),3. Using the Lineweaver-Burk plot determine the values of Km and Vmax.Scan and upload it

Solution The process delineate by the Michaelis–Menten equation will be depicted by a series of…

Answered: Lisa is running an experiment to…

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter11: Chemical Kinetics: Rates Of Reactions

Section: Chapter Questions

Problem 81QRT

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Hello, can someone please help me? I have already hand graphed 1 and 2 and found the v max, however I cannot find the Km value. I am confused because the plots on the linear weaver burk plot do not extend past the values that make up the line. So how am I supposed to estimate the value for the y-intercept in order to find the Km value

Lisa is running an experiment to determine the kinetic parameters (Km and Vmax) of
the enzyme that she is studying. She has run a series of experiments where she
changed the amount of substrate added and measured the accumulation of product
over a 5 second time interval. She used this data to calculate the dependence of the
rate of the reaction on substrate concentration. Her results listed below.
Substrate
Concentration (mM)
Velocity (mM/sec)
2.5
4
6.3
7.6
1
10
20
You need to hand-
1. Using this information construct a Michaelis-Menten curve.
draw on graph
2. Using this information construct a Lineweaver-Burk plot.
paper (no excel),
3. Using the Lineweaver-Burk plot determine the values of Km and Vmax.
Scan and upload it

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