Instructions: This is a take-home problem to help you practice recent material, and you are welcome to work with any Bio 161 students. If you find the distinct roles for group members helpful, you may use this structure and list those roles above. Please upload your answers by 11:59 pm on Tuesday, November 29. PART 1 1. A hypothetical eukaryotic gene with no introns is pictured. (It is much shorter than an actual gene.) The promoter, the initiation site, and the termination site are shown. a. Which strand is the template strand for transcription of this gene? Circle: Promoter Initiation site БА mRNA sequence: 5'-CTATAAAGAGCATGCAITATCTAGATTAACGGTTGCGAC-3' 3'-GATATTTCTCGTACGTAATAGATCTAATTGCCAACGCTG-5' protein sequence: H₂N TOP BOTTOM BOTH b. Based on the DNA template, write the sequence of the bases of the nucleotides that will be incorporated into the mRNA during transcription. Then, label the 5' and 3' ends of the mRNA, the start codon, stop codon, 5' UTR (untranslated region), and 3¹ UTR. Termination site c. Using a codon table (provided on the last page) and your labeled mRNA, determine the order of amino acids in the protein translated from this mRNA. Write the order of amino acids above, using their three letter abbreviations. Label the N-terminus and C-terminus of the protein. ii. Will the resulting protein be different? If so, how? d. A mutation changes the T/A nucleotides in bold and underlined to C/G instead. i. Will the resulting mRNA be different? If so, how?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question B (mRNA sequence and protein sequence)
Instructions: This is a take-home problem to help you practice recent material, and you are welcome
to work with any Bio 161 students. If you find the distinct roles for group members helpful, you may use
this structure and list those roles above. Please upload your answers by 11:59 pm on Tuesday,
November 29.
PART 1
1. A hypothetical eukaryotic gene with no introns is pictured. (It is much shorter than an actual gene.) The
promoter, the initiation site, and the termination site are shown.
a. Which strand is the template strand for transcription of this gene? Circle:
Initiation site
Promoter
5'-CTATAAAGAGCATGCAITATCTAGATTAACGGTTGCGAC-3'
3'-GATATTTCTCGTACGTAATAGATCTAATTGCCAACGCTG-5'
mRNA sequence:
TOP) BOTTOM BOTH
b. Based on the DNA template, write the sequence of the bases of the nucleotides that will be incorporated
into the mRNA during transcription. Then, label the 5' and 3' ends of the mRNA, the start codon, stop
codon, 5' UTR (untranslated region), and 3' UTR.
protein sequence:
H₂N
Termination site
c. Using a codon table (provided on the last page) and your labeled mRNA, determine the order of amino
acids in the protein translated from this mRNA. Write the order of amino acids above, using their three letter
abbreviations. Label the N-terminus and C-terminus of the protein.
ii. Will the resulting protein be different? If so, how?
d. A mutation changes the T/A nucleotides in bold and underlined to C/G instead.
i. Will the resulting mRNA be different? If so, how?
1
Transcribed Image Text:Instructions: This is a take-home problem to help you practice recent material, and you are welcome to work with any Bio 161 students. If you find the distinct roles for group members helpful, you may use this structure and list those roles above. Please upload your answers by 11:59 pm on Tuesday, November 29. PART 1 1. A hypothetical eukaryotic gene with no introns is pictured. (It is much shorter than an actual gene.) The promoter, the initiation site, and the termination site are shown. a. Which strand is the template strand for transcription of this gene? Circle: Initiation site Promoter 5'-CTATAAAGAGCATGCAITATCTAGATTAACGGTTGCGAC-3' 3'-GATATTTCTCGTACGTAATAGATCTAATTGCCAACGCTG-5' mRNA sequence: TOP) BOTTOM BOTH b. Based on the DNA template, write the sequence of the bases of the nucleotides that will be incorporated into the mRNA during transcription. Then, label the 5' and 3' ends of the mRNA, the start codon, stop codon, 5' UTR (untranslated region), and 3' UTR. protein sequence: H₂N Termination site c. Using a codon table (provided on the last page) and your labeled mRNA, determine the order of amino acids in the protein translated from this mRNA. Write the order of amino acids above, using their three letter abbreviations. Label the N-terminus and C-terminus of the protein. ii. Will the resulting protein be different? If so, how? d. A mutation changes the T/A nucleotides in bold and underlined to C/G instead. i. Will the resulting mRNA be different? If so, how? 1
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