In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC, PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in the figure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal from Filter 3 and the PE-Cy 5 signal from Filter 4. Part 4.1 Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal. Part 4.2 You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose 4 and justify your selection. # Filter type Wavelength A Long pass 650 nm B Short pass 530 nm C Long pass 490 nm D Band pass 550-590 nm E Long pass 560 nm F Short pass 640 nm G Long pass 620 nm H Band pass 620-640 nm I Short pass 590 nm

In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC, PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in the figure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal from Filter 3 and the PE-Cy 5 signal from Filter 4. Part 4.1 Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal. Part 4.2 You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose 4 and justify your selection. # Filter type Wavelength A Long pass 650 nm B Short pass 530 nm C Long pass 490 nm D Band pass 550-590 nm E Long pass 560 nm F Short pass 640 nm G Long pass 620 nm H Band pass 620-640 nm I Short pass 590 nm

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter16: Fiber Optics And Lasers In Bioinstrumentation

Section: Chapter Questions

Problem 8Q

See similar textbooks

Similar questions

The usual dose of digoxin for rapid digitalization is a total of 1.0mg, divide into two or more portion at intervals of 6 to 8 hours. How many milliliters of digoxin elixir containing 50mcg/mL would provide this dose?
Milligrams 47.0 milligrams = Multiple Choice 0.47 decigrams 4.7 centigrams 0.047 grams 0.00047 hectograms All of the answer choices are correct.
What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?
good radiograph is taken with 400 mA, 200ms, 16:1 grid find the mAs value if a 6:1 grid is substituted.
Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700
The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
Table of caffeine standards concentration Sample Conc, ppm Std1 16 Std2 32 Std3 48 Std4 64 Std5 80 If the volume used to make 100 mL of std 1 is 2 uL what is the concentration in M used to make a standard calibration curve? The standards are going to be used to build calibration curve to analyze caffeine in an energy drink. If 500 mL of the energy drink has target of 400 mg caffeine, how will you prepare the sample if you need 10 mL for the analysis ? . Caffeine MM=194.19 g/mol.
In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.
Lead nitrate Stock Solution— Dissolve 159.8 mg of lead nitrate in 100 mL of water to which has been added 1 mL of nitric acid, then dilute with water to 1000 mL. Standard Lead Solution— On the day of use, dilute 10.0 mL of Lead Nitrate Stock Solution with water to 100.0 mL. Standard Preparation— Into a 50-mL color-comparison tube pipet 2 mL of Standard Lead Solution, and dilute with water to 40 mL, and mix. Test Preparation— Into a 50-mL color-comparison tube dissolve 1.0g ascorbic acid (mol wt 176.12) in 25 mL of water, dilute with water to 40 mL, and mix. Procedure— To each of the two tubes containing the Standard Preparation, the Test Preparation, add 2 mL of pH 3.5 Acetate Buffer, then add 1.2 mL of thioacetamide–glycerin base TS, dilute with water to 50 mL. Mix, allow to stand for 2 minutes, and view downward over a white surface. The color of the solution from the Test Preparation is not darker than that of the solution from the Standard Preparation. The colour is formed when…
Assume your field of view under a 3X objective is 6mm across. What is the field of view in mm under a 20X objective? Show clear work with units for credit. Field of view 20X objective? ___mm What is the field of view of this 20X objective in micrometers? If you observe a specimen under the 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?
the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.
Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %