In the experiment, imidazole is used to elute mCherry from the column. Instead of using imidazole, can you suggest another method to elute mCherry from the column?
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In the experiment, imidazole is used to elute mCherry from the column. Instead of using imidazole, can you suggest another method to elute mCherry from the column?
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- Prepare 15 mg of meperidine from an ampule containing 50 mg/ ml. How manv ml(s) would be given.?From a 5% normal saline (NaCl) solution, take 2 ml and dilute it 1:5. Take 5 ml of this solution and dilute it 1:10. A 2:100 dilution is made from this last solution. Determine: a) How many times less concentrated is the 2nd dilution than the original? b) What is the dilution factor in each step of the series and what is the final volume of each dilution? c) How many grams of NaCl will be in 1 ml of the last solution?The accompanying figure is from an electrophoresis experiment using SDS–PAGE. The left lane has the following standards: bovine serum albumin (MW 66,000), ovalbumin (MW45,000), glyceraldehyde 3-phosphate dehydrogenase (MW 36,000),carbonic anhydrase (MW 24,000), and trypsinogen (MW 20,000).The right lane is an unknown. Calculate the MW of the unknown.
- You have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose. Please answer with explanation ASAP. I will really upvote. ThanksYou have added 8 mL of Albuterol Sulfate Solution (5mg/mL) and 22 mL of normal saline to your continuous nebulizer with an output of 10 mL/hr. What is the total dosage of the treatment you are giving? How long will this treatment last?An amino acid of interest was produced in a fed-batch culture using glucose in the feed medium. The initial volume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h.
- volume of the quasi-steady-state culture was V0= 500 L, and the nutrient solution containing glucose was added at a constant flow rate of F = 50 L/h.Data: X0 (at the beginning of feeding) = 20 g/L, S0 = 300 g/L, max = 0.2 h-1, KS = 0.5 g/L and Y x/s= 0.3 g/g a) Determine the volume of the culture at t = 10hb) Determine the concentration of glucose at t = 10 hc) determine the concentration and total mass of cells at t = 10 hd) If product is associated with growth with α = 1.5 and P0 = 0.1 g/L, determine the concentration of product at t = 10h. (answer:P = 67,55 g/L)what is the effect and rationale when three milliliters instead of 1 mL was placed in the Petri dish in pour-plate method?Explain this approach: matrix-assisted laser desorption ionization (MALDI) ?
- 50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then a 1:90 dilution is made from this, and then 1 mL from this is added to 99 mL of water, and then 100 uL of this last dilution is plated and after incubation 164 colonies are counted.a) What was the overall dilution factor?b) What was the total CFU/g of the original sample?To prepare a vaginal irrigation, a nurse uses 3 tsp of vinegar and 1.5 L of water. a. How many ml of vinegar should she use? b. If she prepares a solution using only 500 ml of water, how many ml of vinegar should she use?An iodine solution was sealed inside dialysis tubing, then immersed in a cup of water containing fructose and starch and allowed to react. Recall that iodine solution is red, Iodine + starch = purple. After reaction a) the color outside the dialysis bag would be (red, purple, clear) b) the color inside the bag would be purple (true/false) c) fructose would be present inside the dialysis bag (true/false)