In preparation of the ELISA plates, an albumin protein (BSA) was used to "block" the plate after the first antibody was immobilized on the plate. If you skipped that step, would the concentration of IgA appear to be higher (circle the correct answer) lower or the same compared to the actual value? Explain your a answer below.
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Questions:
1. In preparation of the ELISA plates, an albumin protein (BSA) was used to "block" the plate after the first antibody was immobilized on the plate. If you skipped that step, would
the concentration of IgA appear to be higher
(circle the correct answer)
lower or
the same
compared to the actual value? Explain your a answer below.
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- Can you please check and answer the questions that not answered, that is not graded https://www.studocu.com/en-us/ document/university-of-arkansas/microbiology/other/immunology-lab-worksheet-student/7960193/view Questions from ELISA Simulation Introduction Document (posted in Lab Module 8 folder): 1. What term is the word “antigen” derived from? 2. Where are antibodies found in the body? Plasma of the blood 3. In general, what is the cause of an autoimmune disease? 4. What kind of antibodies are present in patients with systemic lupus erythematosus (SLE)? 5. What does ELISA stand for? enzyme-linked immunosorbent assay 6. In general, what is an ELISA test used to detect? to measure the concentration of antibodies or antigens 7. In an indirect ELISA, which antibody is linked to the enzyme? (i.e the primary antibody from the blood serum or the secondary anti-human antibody) Questions from HHMI Virtual Immunology Lab Website Diagnosis…Review Figure 1-8, "Antibody Panel, Case 1-6." In this case study, antibodies are excluded only if the patient's serum does not react with panel cells that are homozygous for the antigen. What is the most likely antibody identified by the antibody panel in Figure 1-8? How would you rule out the remaining antibodiesRewrite the following sentences after correction: 1. The N1303k mutation in the CFTR gene of CF patients is autosomal dominant disorder due to insertion of asparagine at 1303. 2. If a person RBCs have B surface antigen and it will clump with antigen B such clumping indicates blood type B. 3. Indirect ELISA can detect polygenic gene expression.
- Wil completed a full plate titer assay and there were 25 plaques on his 10^-7 plate. What is the titer of his high titer lysate? 10uL of each dilution were used for the plaque assays. O 2.5X10^11 pfu/mL O 2.5X10^7pfu/mL O 2.5X10^9 pfu/mL O 2.5X10^8 pfu/mL O 2.5X10^10 pfu/mLA- B ↑ ↑ C- An image of a hematocrit blood test is shown. Indicate which layer refers to the buffy coat. A B ОсPatient Testing Solution (Mr. Smith) Reaction Phenotype (Blood Type) Possible Genotypes for blood type (Clumping? Antibody-A Antibody-B | Antibody-Rh clumping no clumping clumping A+ AA or AO 1. a) Using a Punnett square determines one possibility of what the patient's (Mr. Smith) parent's blood types could be. For the Punnett square, ignore the Rh+ and Rh-. b) Could the parents be a possible donor? Explain why or why not? You may consider Rh+ and Rh- here. c)Which donor would you recommend, if any? Explain your choice using concepts and vocabulary from the course. You may use words, drawings or diagrams to clarify your choice. (Approximately 1 paragraph in length). 20 tv MacBook Pro & %23 $ % 2 3 4 7 E R Y P D F K C V 36 MOSISO comm nd
- Sample Anti -A Anti-B Anti - D Blood type 1 Clumping Clear Clear A- Clear Clumping Clumping Clear B- Clumping Clear 3 Clear AB- 4 Clear Clear 0- 2. Explain why clotting did or did not occur with each antibody in each sample. a. Sample 1 b. Sample 2 c. Sample 3 d. Sample 4 3. What is the antibody finding on the red blood cells? 4. What blood type is the universal donor? Why? 5. What blood type is the universal recipient? Why? 6. Can Blood Type A-be safelygiven to a person with blood type B+? Why/Why Not; Explain English (United States) EFocus E E -.Phenotype: A2B 1. Give the possible genotype/s of A:B 2. If you were to perform the blood typing of this individual, what are the expected results given the following reagents Phenotype Forward typing (reagents) Reverse typing (reagents) Anti-A Anti-B Anti-H A cells B cells Al Write "+" for presence of agglutination and "-" for absence of agglutination 3. Explain the results of the blood typing, why would there be a "+" or a "" reaction during the testing. Relate your answer to the presence/absence of antigens and/or antibodiesThe technician decided to antigen type the patient to confirm predictions from the antibody panel. Antigen type results are given below. Given these results, which of the following statements is BEST supported? Rh Kell Duffy Kidd anti-D anti-C anti-E anti-c anti-e anti-K anti-Fya anti-Fyb anti-Jka anti-Jkb 3+ 0 3+ 3+ 0 0 0 3+ 2+ 2+ Question 7 options: A) K and Fya antibodies are likely because patient would not readily produce antibodies against their own cells. B) An anti-e antibody must be present due to the lack of antigen on the patient cells. C) Jka and Jkb antibodies are likely because an autoantibody is present. D) Rh antibodies are likely because the patient is Rh negative.
- What is a screening test? Immunoassay Enzyme immunoassay (EAI) Enzyme-multiplied immunoassay technique (EMIT) Fluorescence polarization Radioimmunoassay (RIA) Chromatography Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC) What is a confirmatory test? Hyphenated technique. Combination of two sophisticated technologies (I.e., Gas Chromatography - mass spectrometry or GC-MS) or other modern and acceptable techniques (l.e., LC-MS, GC-MS-MS, or LC-MS-MS).BIO152L Worksheet for Howard Hughes Medical Institute: The Virtual Immunology Lab Name: Student ID number: Questions from ELISA Simulation Introduction Document (posted in Lab Module 8 folder): 1. What term is the word “antigen” derived from? 2. Where are antibodies found in the body? 3. In general, what is the cause of an autoimmune disease? 4. What kind of antibodies are present in patients with systemic lupus erythematosus (SLE)? 5. What does ELISA stand for? 6. In general, what is an ELISA test used to detect? 7. In an indirect ELISA, which antibody is linked to the enzyme? (i.e the primary antibody from the blood serum or the secondary anti-human antibody) Questions from HHMI Virtual Immunology Lab Website Diagnosis (The answers to the following question can be found in the “Diagnosis” tab) 8. What are the 3 important limitations of an ELISA? Explain each.…When an enzyme-linked antibody is added in an ELISA test (select all appropriate), Group of answer choices A. it will be washed away if the plasma sample is positive for the substance we are testing for B. It will attach to the substance we are testing for if the sample is positive C. it will stick if the plasma sample is positive for the substance we are testing for